Mathieu-Costello O
Microvasc Res. 1987 Jan;33(1):98-117. doi: 10.1016/0026-2862(87)90010-0.
The effect of muscle contraction, and extension, on capillary anisotropy was investigated in rat m. soleus fixed by vascular perfusion at sarcomere lengths ranging from 1.62 micron (tetanizing stimulation of sciatic nerve) to 2.85 micron (ankle joint maximally flexed. Capillary length density and tortuosity were estimated by morphometry using two sets of sections (0 and 90 degrees to the fiber axis). Capillary orientation distribution was evaluated from a series of sections taken at 0 to 90 degrees (by steps of 5-10 degrees) to the fiber axis in six preparations (sarcomere length range, 1.62-2.85 micron; capillary length density, 900-2000 mm-2). The Fisher axial distribution provided a good fit for modeling capillary orientation distribution in each case. For a comparable capillary length density per volume of muscle fiber (approximately equal to 2000 mm-2), the degree of orientation of capillary segments parallel to the fiber axis was two and four times larger in extended m. soleus than in the muscles fixed at 1.98- and 1.62-micron sarcomere lengths, respectively. In preparations fixed at 2.85, 1.98, and 1.62 micron, capillary length density per volume of muscle fiber was, respectively, 14, 44, and 65% larger than revealed by capillary counts per sectional area of muscle fiber on transverse section only, an often used parameter to compare capillarity in different muscles.
通过血管灌注固定大鼠比目鱼肌，在肌节长度从1.62微米（坐骨神经强直刺激）到2.85微米（踝关节最大程度屈曲）的范围内，研究了肌肉收缩和伸展对毛细血管各向异性的影响。使用两组与纤维轴呈0度和90度的切片，通过形态测量法估计毛细血管长度密度和曲折度。在六个标本（肌节长度范围为1.62 - 2.85微米；毛细血管长度密度为900 - 2000 mm-2）中，从与纤维轴呈0度至90度（步长为5 - 10度）的一系列切片评估毛细血管取向分布。费舍尔轴向分布在每种情况下都能很好地拟合毛细血管取向分布模型。对于每单位体积肌纤维具有可比的毛细血管长度密度（约等于2000 mm-2），在伸展的比目鱼肌中，平行于纤维轴的毛细血管段的取向程度分别比在肌节长度为1.98微米和1.62微米固定的肌肉中大四倍和两倍。在肌节长度为2.85、1.98和1.62微米固定的标本中，每单位体积肌纤维的毛细血管长度密度分别比仅通过肌肉纤维横截面积上的毛细血管计数所显示的大14%、44%和65%，而后者是常用于比较不同肌肉毛细血管化的参数。
