College of Life Sciences and Engineering, Foshan University, Foshan 528225, Guangdong Province, PR China.
College of Life Sciences and Engineering, Foshan University, Foshan 528225, Guangdong Province, PR China.
J Inorg Biochem. 2022 Aug;233:111838. doi: 10.1016/j.jinorgbio.2022.111838. Epub 2022 Apr 20.
Nanosilver is a metallic silver monomer with a diameter of <100 nm, which has excellent antibacterial activity and is widely used in the fields of medicine and sanitation, disinfection of drinking water in daily life and feed additives in livestock and poultry farming. Heterophil extracellular traps (HETs) are an important part of innate immunity in chickens and have an excellent antimicrobial effect, but their excessive release caused tissue damage. Nanosilver overdose caused toxic effects in chickens, while immunotoxic effects of nanosilver on chickens have not been reported. In this study, we explored the effects of nanosilver-induced HETs on chicken liver and kidney damage and further investigated the molecular mechanism of nanosilver-induced HETs release. The results showed that nanosilver significantly upregulated serum HETs and caused liver and kidney damage. The classical structure of nanosilver-induced HETs was also observed, and nanosilver-induced HETs were dependent on reactive oxygen species (ROS), extracellular regulatory protein kinase (ERK), p38 and glycolysis pathways. In summary, this research suggests that nanosilver induced HETs release, but excessive HETs release also caused damage to chicken. It also helps to understand the importance of moderate application of nanosilver, which may improve animal immunity but avoid negative effects in safeguarding the economic efficiency of poultry farming.
纳米银是一种直径小于 100nm 的金属银单体，具有优异的抗菌活性，广泛应用于医学和卫生领域、日常生活饮用水消毒以及畜禽养殖中的饲料添加剂。嗜中性粒细胞细胞外诱捕网（HETs）是鸡固有免疫的重要组成部分，具有出色的抗菌作用，但它们的过度释放会导致组织损伤。纳米银过量会对鸡造成毒性作用，而纳米银对鸡的免疫毒性作用尚未有报道。在本研究中，我们探讨了纳米银诱导的 HETs 对鸡肝、肾损伤的影响，并进一步研究了纳米银诱导的 HETs 释放的分子机制。结果表明，纳米银显著上调血清 HETs 水平，导致肝、肾损伤。还观察到了纳米银诱导的 HETs 的经典结构，并且纳米银诱导的 HETs 依赖于活性氧（ROS）、细胞外调节蛋白激酶（ERK）、p38 和糖酵解途径。综上所述，本研究表明纳米银诱导了 HETs 的释放，但过量的 HETs 释放也会对鸡造成损伤。这也有助于理解适度应用纳米银的重要性，适度应用纳米银可能会提高动物免疫力，但在保障家禽养殖经济效益的同时避免负面影响。
