Department of Biostatistics and Bioinformatics, Emory University Rollins School of Public Health, Atlanta, GA, USA.
Department of Human Genetics, Emory University School of Medicine, Atlanta, GA, USA.
Methods Mol Biol. 2022;2432:211-226. doi: 10.1007/978-1-0716-1994-0_16.
Bisulfite sequencing (BS-seq) technology has enabled the detection and measurement of DNA methylation at the single-nucleotide level. A fundamental question in functional epigenomics research is whether DNA methylation varies under different biological contexts. Thus, identifying differentially methylated loci/regions (DML/DMRs) is a key task in BS-seq data analysis. Here we describe detailed procedures to perform differential methylation analyses for BS-seq using the Bioconductor package DSS. The analysis scheme in this chapter will guide researchers through differential methylation analyses by providing step-by-step instructions for analytical tools.
亚硫酸氢盐测序（BS-seq）技术使在单核苷酸水平检测和测量 DNA 甲基化成为可能。功能表观基因组学研究中的一个基本问题是 DNA 甲基化是否在不同的生物背景下发生变化。因此，鉴定差异甲基化基因座/区域（DML/DMRs）是 BS-seq 数据分析中的关键任务。在这里，我们使用 Bioconductor 包 DSS 描述了用于 BS-seq 的差异甲基化分析的详细过程。本章中的分析方案将通过为分析工具提供逐步说明，指导研究人员进行差异甲基化分析。
