Central Institute for Supervising and Testing in Agriculture, Division of Plant Pest Diagnostics, Šlechtitelů 773/23, 779 00 Olomouc, Czech Republic.
Faculty of Forestry and Wood Technology, Department of Forest Protection and Game Management, Mendel University in Brno, Zemědělská 3, 613 00 Brno, Czech Republic.
J Helminthol. 2022 May 4;96:e31. doi: 10.1017/S0022149X22000141.
Two populations of the species Diastolaimus grossus have been obtained from bark of trees in Bosnia and Herzegovina and the Czech Republic. The species is described and characterized in detail using morphological techniques (light and electron scanning microscopy) and morphometrical (Gower General Similarity coefficient of morphological characters) and molecular analyses, including phylogenetic analysis of all related and already sequenced species of the family Chambersiellidae. Morphological and molecular analyses, based on 18S and 28S ribosomal DNA sequences, show that the family Chambersiellidae is polyphyletic, being the subfamily Chambersiellinae related with Cephalobomorpha and Tylenchomorpha, and the subfamily Macrolaiminae is located into Panagrolaimomorpha. The genus Diastolaimus, previously belonging to Macrolaiminae, is transferred to Chambersiellinae. Diastolaimus mexicanus is proposed as a junior synonym of D. grossus.
已经从波斯尼亚和黑塞哥维那及捷克共和国的树木树皮中获得了两个大杆属(Diastolaimus)种群。该物种使用形态学技术(光镜和电子扫描显微镜)和形态计量学(形态特征的戈尔总体相似系数)以及分子分析进行了详细描述和特征分析,包括对亲缘关系密切且已测序的 Chambersiellidae 科所有相关物种的系统发育分析。基于 18S 和 28S 核糖体 DNA 序列的形态和分子分析表明,Chambersiellidae 科是多系的,Chambersiellinae 亚科与 Cephalobomorpha 和 Tylenchomorpha 有关,而 Macrolaiminae 亚科位于 Panagrolaimomorpha 中。先前属于 Macrolaiminae 的 Diastolaimus 属被转移到 Chambersiellinae。建议将 Diastolaimus mexicanus 作为 D. grossus 的次异名。