[Effects of SIN-1 on the metabolism of arachidonic acid in vascular smooth muscle cells in culture].

The effects of SIN-1 on the metabolism of Eicosanoids (prostaglandins, hydroxylated derivatives) were investigated in cultured arterial smooth muscle cells. These cells can use arachidonic acid in two different metabolic pathways involving cyclooxygenase and lipoxygenases activities and leading to the formation of prostaglandins (mainly PGI2) and hydroxylated derivatives (HHT and 11, 15, 12 and 5 HETES). Results shows that SIN-1 produces no modification in PGI2 production, whether under baseline conditions or in the presence of activators for the cell type under study. SIN-1 seems to have no direct action on the sequence of enzyme activities (cyclooxygenase, lipoxygenases). However, SIN-1 significantly alters the distribution of 14C arachidonic acid within membrane structures.