Centre for Biomedical Research, Faculty of Medicine, University of Banja Luka, Banja Luka, The Republic of Srpska 78000, Bosnia and Herzegovina.
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Belgrade, Belgrade 11 000, Serbia.
Can J Physiol Pharmacol. 2022 Aug 1;100(8):755-762. doi: 10.1139/cjpp-2021-0736. Epub 2022 May 4.
Carvacrol (CRV) is the main compound of essential oils extracted primarily from and species. Its various biological activities were confirmed: antioxidant, anti-inflammatory, antibacterial, antifungal, anti-tumour, antinematodal, and vasorelaxant action. Although vasodilation mediated by CRV was previously described, the exact mechanism of its action has not yet been established. Hence, the aim of this study was to investigate CRV vasoactivity on human umbilical arteries (HUA) and the different pathways involved in its mechanism of action using the tissue bath methodology. CRV caused a significant decrease in vascular tension of 5-HT-pre-contracted umbilical arteries, with EC of 442.13 ± 33.8 µmol/L (mean ± standard error of the mean-SEM). At 300 µmol/L, CRV shifted downward the 5-HT concentration-response curve with a statistical significance of < 0.001 obtained for the four highest concentrations. At a concentration of 1 mmol/L, CRV completely abolished BaCl-induced contraction in Ca-free Krebs-Ringer bicarbonate solution and the BAY K 8644-induced contraction in Krebs-Ringer bicarbonate solution ( < 0.001). Isopentenyl pyrophosphate, the antagonist of TRPV channel, was able to decrease the efficacy of CRV ( < 0.001). The blocking of L-type Ca channels on smooth muscle cells is the most probable mechanism of CRV-induced vasorelaxation. However, the role of TRPV channels in CRV-induced vasodilation of HUA cannot be excluded either.
香芹酚（CRV）是主要从牛至和迷迭香属植物中提取的精油的主要化合物。其具有多种生物活性已被证实：抗氧化、抗炎、抗菌、抗真菌、抗肿瘤、抗线虫和血管舒张作用。尽管先前已经描述了由 CRV 介导的血管舒张作用，但它的确切作用机制尚未确定。因此，本研究旨在使用组织浴方法研究 CRV 对人脐动脉（HUA）的血管活性及其作用机制中涉及的不同途径。CRV 导致 5-HT 预收缩的脐动脉血管张力显着下降，EC 为 442.13 ± 33.8 µmol/L（平均值 ± 标准误差均值-SEM）。在 300 µmol/L 时，CRV 向下移动 5-HT 浓度反应曲线，四个最高浓度的统计意义均为 <0.001。在 1 mmol/L 时，CRV 完全消除了 Ca 自由 Krebs-Ringer 碳酸氢盐溶液中的 BaCl 诱导收缩和 Krebs-Ringer 碳酸氢盐溶液中的 BAY K 8644 诱导收缩（<0.001）。TRPV 通道拮抗剂异戊烯焦磷酸能够降低 CRV 的功效（<0.001）。平滑肌细胞上 L 型 Ca 通道的阻断是 CRV 诱导血管舒张的最可能机制。然而，也不能排除 TRPV 通道在 CRV 诱导的 HUA 血管舒张中的作用。
