抗sortilin单克隆抗体诱导人乳腺癌细胞凋亡。

Monoclonal Antibody Against Sortilin Induces Apoptosis in Human Breast Cancer Cells.

作者信息

Simonian Miganoosh, Haji Ghaffari Mozhan, Salimi Ali, Mirzadegan Ebrahim, Sadeghi Niloufar, Ebrahimnezhad Nasim, Fazli Ghazaleh, Fatemi Ramina, Bayat Ali-Ahmad, Milani Saeideh, Negahdari Babak, Rabbani Hodjattallah

机构信息

Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.

出版信息

Avicenna J Med Biotechnol. 2022 Jan-Mar;14(1):37-45. doi: 10.18502/ajmb.v14i1.8168.

DOI:10.18502/ajmb.v14i1.8168

PMID:35509360

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9017463/

Abstract

BACKGROUND

Sortilin has an important role in various malignances and can be used as a promising target to eradicate cancer cells.

METHODS

In this study, the expression of sortilin in 4T1 and MDA-MB231 cell lines was evaluated by flow cytometry and immunocytochemistry. Apoptosis assay was also applied to evaluate apoptosis induction in 4T1 and MDA-MB231 cell lines.

RESULTS

Based on cell surface flow cytometry results, anti-sortilin (2D8-E3) mAb could recognize sortilin molecules in 79.2% and 90.3% of 4T1 and MDA-MB231 cell-lines, respectively. The immunocytochemistry staining results confirmed sortilin surface expression. Apoptosis assay indicated that anti-sortilin mAb could induce apoptosis in 4T1 and MDA-MB231 cell lines.

CONCLUSION

Our study revealed the important role of surface sortilin in breast carcinoma cell survival and its possible application as a therapeutic agent in cancer targeted therapies.

摘要

背景

Sortilin在多种恶性肿瘤中发挥重要作用，可作为根除癌细胞的一个有前景的靶点。

方法

在本研究中，通过流式细胞术和免疫细胞化学评估Sortilin在4T1和MDA-MB231细胞系中的表达。还应用凋亡检测来评估4T1和MDA-MB231细胞系中的凋亡诱导情况。

结果

基于细胞表面流式细胞术结果，抗Sortilin（2D8-E3）单克隆抗体可分别识别4T1和MDA-MB231细胞系中79.2%和90.3%的Sortilin分子。免疫细胞化学染色结果证实了Sortilin的表面表达。凋亡检测表明抗Sortilin单克隆抗体可诱导4T1和MDA-MB231细胞系凋亡。