Centre for Microsystems Technology (CMST), Imec and Ghent University, Technologiepark Zwijnaarde 126, 9052 Zwijnaarde, Ghent, Belgium.
Reproductive Biology Unit (RBU), Faculty of Veterinary Medicine, Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, Salisburylaan 133 D4 entrance 4, 9820 Merelbeke, Belgium.
Lab Chip. 2022 May 17;22(10):1852-1875. doi: 10.1039/d1lc01160j.
Electrical stimulation of gametes and embryos and on-chip manipulation of microdroplets of culture medium serve as promising tools for assisted reproductive technologies (ARTs). Thus far, dielectrophoresis (DEP), electrorotation (ER) and electrowetting on dielectric (EWOD) proved compatible with most laboratory procedures offered by ARTs. Positioning, entrapment and selection of reproductive cells can be achieved with DEP and ER, while EWOD provides the dynamic microenvironment of a developing embryo to better mimic the functions of the oviduct. Furthermore, these techniques are applicable for the assessment of the developmental competence of a mammalian embryo . Such research paves the way towards the amelioration and full automation of the assisted reproduction methods. This article aims to provide a summary on the recent developments regarding electrically stimulated lab-on-chip devices and their application for the manipulation of gametes and embryos .
电刺激配子和胚胎以及微滴在芯片上的操作是辅助生殖技术 (ARTs) 的有前途的工具。到目前为止，介电泳 (DEP)、电旋转 (ER) 和电润湿 (EWOD) 与 ARTs 提供的大多数实验室程序兼容。通过 DEP 和 ER 可以实现生殖细胞的定位、捕获和选择，而 EWOD 则为胚胎的发育提供了动态微环境，更好地模拟了输卵管的功能。此外，这些技术适用于评估哺乳动物胚胎的发育能力。这些研究为辅助生殖方法的改进和完全自动化铺平了道路。本文旨在提供有关电刺激芯片实验室设备的最新进展及其在配子和胚胎操作中的应用的概述。
