研究 Chordin 样蛋白 1 在口腔鳞状细胞癌中的表达及功能。

Study on the expression and function of chordin-like 1 in oral squamous cell carcinoma.

机构信息

Department of Oral and Maxillofacial Surgery, Center of Stomatology, Xiangya Hospital of Central South University, Changsha, Hunan, China.

Center for Medical Genetics & Hunan Key Laboratory of Medical Genetics, School of Life Sciences, Central South University, Changsha, Hunan, China.

出版信息

Oral Dis. 2023 Jul;29(5):2034-2051. doi: 10.1111/odi.14240. Epub 2022 May 17.

DOI:10.1111/odi.14240

PMID:35510812

Abstract

OBJECTIVE

The aim of this study was to investigate the role and related mechanism of chordin-like 1 (CHRDL1) in oral squamous cell carcinoma (OSCC).

METHODS

The expressions of CHRDL1 were analyzed in both mRNA and protein levels by bioinformatics analysis, immunohistochemistry, and fluorescence in situ hybridization in OSCC. Survival analysis was used to determine the relationship between CHRDL1 and prognosis. In addition, enrichment analysis was used to suggest signal pathways involved in CHRDL1. Besides, the relationships between CHRDL1 and miRNAs, hypoxia, and immune infiltration were explored.

RESULTS

The mRNA level of CHRDL1 in OSCC was significantly lower than that in normal tissues, while the protein level was significantly higher than that in normal tissues. The high mRNA levels of CHRDL1 suggested a poor prognosis in patients with OSCC. The enrichment results showed that CHRDL1 might be involved in the Calcium signaling pathway, dilated cardiomyopathy, and focal adhesion. 7 immune cells were positively correlated with CHRDL1, while Tgd was negatively correlated with CHRDL1. In addition, we also found that hsa-miR-455-3p directly targeted CHRDL1 and reduced the mRNA levels of CHRDL1.

CONCLUSION

CHRDL1 plays a vital role in promoting cancer in OSCC and is down-regulated at the mRNA levels by hsa-miR-455-3p.

摘要

目的

本研究旨在探讨 Chordin-like 1（CHRDL1）在口腔鳞状细胞癌（OSCC）中的作用及相关机制。

方法

通过生物信息学分析、免疫组织化学和荧光原位杂交技术，分析 CHRDL1 在 OSCC 中的 mRNA 和蛋白水平表达。生存分析用于确定 CHRDL1 与预后的关系。此外，富集分析用于提示 CHRDL1 涉及的信号通路。还探索了 CHRDL1 与 miRNAs、缺氧和免疫浸润之间的关系。

结果

CHRDL1 在 OSCC 中的 mRNA 水平明显低于正常组织，而蛋白水平明显高于正常组织。CHRDL1 的高 mRNA 水平提示 OSCC 患者预后不良。富集结果表明，CHRDL1 可能参与钙信号通路、扩张型心肌病和黏着斑。7 种免疫细胞与 CHRDL1 呈正相关，而 Tgd 与 CHRDL1 呈负相关。此外，我们还发现 hsa-miR-455-3p 可直接靶向 CHRDL1，降低 CHRDL1 的 mRNA 水平。