Department of Ophthalmology, the Second Affiliated Hospital of Army Medical University, Chongqing, PR China.
Proteomics Clin Appl. 2022 Sep;16(5):e2100128. doi: 10.1002/prca.202100128. Epub 2022 May 11.
The purpose of the present study was to characterize the idiopathic epiretinal membrane (iERM) through proteomics and phosphoproteomics analysis to facilitate the diagnosis and treatment of iERM.
The vitreous of 25 patients with an iERM and 15 patients with an idiopathic macular hole were analyzed by proteomic and phosphoproteomic analysis based on tandem mass tag. PRM was used to verify the differential proteins.
Proteomic analysis identified a total of 878 proteins, including 50 differential proteins. Tenascin-C, galectin-3-binding protein, glucose-6-phosphate isomerase, neuroserpin, collagen alpha-1(XI) chain, and collagen alpha-1(II) chain were verified to be upregulated in iERM by PRM. Phosphoproteomic analysis identified a total of 401 phosphorylation sites on 213 proteins, including 27 differential phosphorylation sites on 24 proteins. Mitogen-activated protein kinase-activated protein kinase (MAPKAPK)3 and MAPKAPK5 were predicted as the major kinases in the vitreous of iERM. Twenty-six of the differential proteins and phosphorylated proteins may be closely related to fibrosis in iERM.
Our results indicated the potential biomarkers or therapeutic targets for iERM, provided key kinases that may be involved in iERM. Fibrosis plays an essential role in iERM, and further exploration of related differential proteins has important clinical significance.
本研究旨在通过蛋白质组学和磷酸化蛋白质组学分析对特发性视网膜内膜（iERM）进行特征分析，以促进 iERM 的诊断和治疗。
本研究基于串联质量标签对 25 例 iERM 患者和 15 例特发性黄斑裂孔患者的玻璃体进行了蛋白质组学和磷酸化蛋白质组学分析。采用 PRM 来验证差异蛋白。
蛋白质组学分析共鉴定出 878 种蛋白质，包括 50 种差异蛋白。Tenascin-C、半乳糖凝集素-3 结合蛋白、葡萄糖-6-磷酸异构酶、神经丝氨酸蛋白酶、胶原α-1(XI)链和胶原α-1(II)链通过 PRM 被验证在 iERM 中上调。磷酸化蛋白质组学共鉴定出 213 种蛋白质上的 401 个磷酸化位点，包括 24 种蛋白质上的 27 个差异磷酸化位点。丝裂原活化蛋白激酶激活的蛋白激酶（MAPKAPK）3 和 MAPKAPK5 被预测为 iERM 玻璃体中的主要激酶。26 个差异蛋白和磷酸化蛋白可能与 iERM 中的纤维化密切相关。
我们的结果表明了 iERM 的潜在生物标志物或治疗靶点，为 iERM 可能涉及的关键激酶提供了依据。纤维化在 iERM 中起着重要作用，进一步探索相关差异蛋白具有重要的临床意义。
