J Vis Exp. 2022 May 5(183). doi: 10.3791/6499.
An erratum was issued for: Stereotaxic Viral Injection and Gradient-Index Lens Implantation for Deep Brain In Vivo Calcium Imaging. The Discussion was updated. The following paragraph was added to the end of the Discussion: Although a chunk of brain tissue is removed unilaterally during the GRIN lens implantation step, we did not observe any obvious behavior deficits . The weight of the miniscope is around 2 grams and the cable is custom-designed to make it light and to ensure that the mouse can easily carry it. The miniscope and cable are only attached to the animal prior to in vivo imaging and detached after imaging. The entire imaging process usually takes no longer than 30 minutes. Therefore, these instrumentations do not prevent the mouse from freely behaving. The miniscope installation and deinstallation steps need a brief anesthesia (less than 2 minutes) with isoflurane for the purpose of animal restraining. We typically let the mouse recover from the brief exposure of isoflurane for 30 minutes before performing in vivo imaging. We have performed miniscope in vivo calcium imaging once per week for a few weeks without noticing any impact on mouse health and mouse social behavior.
立体定位病毒注射和梯度折射率透镜植入术用于活体深部脑钙成像。讨论部分更新。在讨论部分的结尾添加了以下段落：虽然在梯度折射率透镜植入步骤中会单侧切除一部分脑组织，但我们没有观察到任何明显的行为缺陷。微型显微镜的重量约为 2 克，电缆是专门设计的，使其轻便，并确保老鼠可以轻松携带。微型显微镜和电缆仅在活体成像前连接到动物身上，成像后断开。整个成像过程通常不超过 30 分钟。因此，这些仪器不会妨碍老鼠自由活动。微型显微镜的安装和拆卸步骤需要短暂的异氟烷麻醉（不到 2 分钟）以固定动物。我们通常让老鼠在短暂暴露于异氟烷后恢复 30 分钟，然后再进行活体成像。我们已经每周进行一次微型显微镜活体钙成像，持续数周，没有发现对老鼠健康和社交行为的任何影响。
