Zhang Yingying, Zhang Chao, Wu Yingnan, Zhao Bing, Wang Liyan, Song Bo
College of Chemistry and Chemical Engineering, Qiqihar University Qiqihar 161006 Heilongjiang China
Institute of Molecular Sciences and Engineering, Shandong University Qingdao 266237 Shandong China.
RSC Adv. 2019 Jul 29;9(40):23382-23389. doi: 10.1039/c9ra03924d. eCollection 2019 Jul 23.
A water-soluble and colorimetric fluorescent probe with a large Stokes shift (139 nm) for rapidly detecting Hg, namely Hcy-mP, was synthesized by using an indole derivative and 2,4-dihydroxybenzaldehyde as starting materials. This probe demonstrates good selectivity for Hg over other metal ions including Ag, Pb, Cd, Cr, Zn, Fe, Co, Ni, Cu, K, Na, Mg, and Ca in aqueous solution. With the increase in concentration of Hg, the color of the solution changed from pale yellow to pink and the fluorescence intensity decreased slightly. When 5-equivalents of EDTA were added to the solution with Hg, the fluorescence intensity of this probe was restored. The probe has been applied to the detection of Hg in real water samples. Moreover, this probe was confirmed to have low cytotoxicity and excellent cell membrane permeability. The effect of Hcy-mP-Hg towards living cells by confocal fluorescence was also investigated.
以吲哚衍生物和2,4 - 二羟基苯甲醛为原料合成了一种用于快速检测汞的具有大斯托克斯位移（139 nm）的水溶性比色荧光探针，即Hcy - mP。该探针在水溶液中对汞表现出优于其他金属离子（包括银、铅、镉、铬、锌、铁、钴、镍、铜、钾、钠、镁和钙）的良好选择性。随着汞浓度的增加，溶液颜色从浅黄色变为粉红色，荧光强度略有下降。当向含汞溶液中加入5当量的乙二胺四乙酸（EDTA）时，该探针的荧光强度得以恢复。该探针已应用于实际水样中汞的检测。此外，该探针被证实具有低细胞毒性和优异的细胞膜通透性。还研究了Hcy - mP - Hg对活细胞的共聚焦荧光效应。
