• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

存在于[-PS]-(DNA#LNA)嵌合体中的锁核酸（LNA）单元增强了与（2'-O-甲基）-RNA链形成的平行双链体和三链体的热稳定性。

LNA units present in [ -PS]-(DNA#LNA) chimeras enhance the thermal stability of parallel duplexes and triplexes formed with (2'-OMe)-RNA strands.

作者信息

Jastrzębska Katarzyna, Mikołajczyk Barbara, Guga Piotr

机构信息

Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences Sienkiewicza 112 90-363 Łódź Poland

出版信息

RSC Adv. 2020 Jun 10;10(38):22370-22376. doi: 10.1039/d0ra03934a.

DOI:10.1039/d0ra03934a
PMID:35514591
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9054617/
Abstract

The results of CD measurements indicate that 2-4 LNA units distributed along 12 nt P-stereodefined phosphorothioate [ -PS]-(DNA#LNA) chimeras impose a C3'- conformation on the 2'-deoxyribonucleosides. Under neutral and slightly acidic conditions homopurine [ -PS]-(DNA#LNA) hybridizes with 9-12 nt Hoogsteen-paired (2'-OMe)-RNA strands to form parallel duplexes, which are thermally more stable than the reported earlier analogous complexes containing LNA-free [ -PS]-DNA oligomers (Δ = 7 °C per LNA unit at pH 5.4). Upon addition of the corresponding Watson-Crick-paired (2'-OMe)-RNA strands, parallel triplexes are formed with further increased thermal stability.

摘要

圆二色光谱测量结果表明，沿着12个核苷酸的P-立体定向硫代磷酸酯[-PS]-(DNA#LNA)嵌合体分布的2 - 4个LNA单元会使2'-脱氧核糖核苷呈现C3'-构象。在中性和略酸性条件下，同型嘌呤[-PS]-(DNA#LNA)与9 - 12个核苷酸的Hoogsteen配对(2'-OMe)-RNA链杂交形成平行双链体，其热稳定性比早期报道的不含LNA的[-PS]-DNA寡聚物的类似复合物更高（在pH 5.4时，每个LNA单元的Δ = 7℃）。加入相应的沃森-克里克配对(2'-OMe)-RNA链后，会形成热稳定性进一步提高的平行三链体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/9054617/ed170e3505ee/d0ra03934a-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/9054617/a2e6cb1386c3/d0ra03934a-c1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/9054617/fe22f17059c3/d0ra03934a-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/9054617/467a60fe9dfc/d0ra03934a-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/9054617/cc4d15954227/d0ra03934a-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/9054617/04277d89ea98/d0ra03934a-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/9054617/abe94bf199b6/d0ra03934a-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/9054617/ed170e3505ee/d0ra03934a-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/9054617/a2e6cb1386c3/d0ra03934a-c1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/9054617/fe22f17059c3/d0ra03934a-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/9054617/467a60fe9dfc/d0ra03934a-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/9054617/cc4d15954227/d0ra03934a-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/9054617/04277d89ea98/d0ra03934a-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/9054617/abe94bf199b6/d0ra03934a-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/9054617/ed170e3505ee/d0ra03934a-f5.jpg

相似文献

1
LNA units present in [ -PS]-(DNA#LNA) chimeras enhance the thermal stability of parallel duplexes and triplexes formed with (2'-OMe)-RNA strands.存在于[-PS]-(DNA#LNA)嵌合体中的锁核酸（LNA）单元增强了与（2'-O-甲基）-RNA链形成的平行双链体和三链体的热稳定性。
RSC Adv. 2020 Jun 10;10(38):22370-22376. doi: 10.1039/d0ra03934a.
2
LNA units present in the (2'-OMe)-RNA strand stabilize parallel duplexes (2'-OMe)-RNA/[All-R(P)-PS]-DNA and parallel triplexes (2'-OMe)-RNA/[All-R(P)-PS]-DNA/RNA. An improved tool for the inhibition of reverse transcription.在（2'-O-甲基）RNA 链中存在的 LNA 单元稳定平行双链（2'-O-甲基）RNA/[All-R(P)-PS]-DNA 和平行三聚体（2'-O-甲基）RNA/[All-R(P)-PS]-DNA/RNA。一种改进的逆转录抑制工具。
Org Biomol Chem. 2015 Feb 28;13(8):2375-84. doi: 10.1039/c4ob02364a.
3
Homopurine R-stereodefined phosphorothioate analogs of DNA with hampered Watson-Crick base pairings form Hoogsteen paired parallel duplexes with (2'-OMe)-RNAs.具有受阻 Watson-Crick 碱基配对的 DNA 的同嘌呤 R-立体限定硫代磷酸酯类似物与 (2'-OMe)-RNAs 形成 Hoogsteen 配对的平行双链体。
Org Biomol Chem. 2019 May 8;17(18):4611-4620. doi: 10.1039/c8ob03112f.
4
Thermal stability and conformation of antiparallel duplexes formed by P-stereodefined phosphorothioate DNA/LNA chimeric oligomers with DNA and RNA matrices.由具有DNA和RNA基质的P-立体定向硫代磷酸酯DNA/LNA嵌合寡聚物形成的反平行双链体的热稳定性和构象。
Org Biomol Chem. 2015 Oct 21;13(39):10032-40. doi: 10.1039/c5ob01474c.
5
Synthesis and hybridizing properties of P-stereodefined chimeric [PS]-{DNA:RNA} and [PS]-{DNA:(2'-OMe)-RNA} oligomers.P-立体定向嵌合[PS]-{DNA:RNA}和[PS]-{DNA:(2'-O-甲基)-RNA}寡聚物的合成及杂交特性
RSC Adv. 2022 Sep 21;12(41):26815-26824. doi: 10.1039/d2ra04855h. eCollection 2022 Sep 16.
6
Hoogsteen-paired homopurine [RP-PS]-DNA and homopyrimidine RNA strands form a thermally stable parallel duplex.Hoogsteen配对的同型嘌呤[RP-PS]-DNA与同型嘧啶RNA链形成热稳定的平行双链体。
Biophys J. 2007 Nov 15;93(10):3567-74. doi: 10.1529/biophysj.107.108183. Epub 2007 Aug 10.
7
Unusual thermal stability of RNA/[RP-PS]-DNA/RNA triplexes containing a homopurine DNA strand.包含同型嘌呤DNA链的RNA/[RP-PS]-DNA/RNA三链体异常的热稳定性。
Biophys J. 2007 Apr 1;92(7):2507-15. doi: 10.1529/biophysj.106.099283. Epub 2007 Jan 11.
8
Evidence from CD spectra and melting temperatures for stable Hoogsteen-paired oligomer duplexes derived from DNA and hybrid triplexes.来自圆二色光谱和熔解温度的证据，关于源自DNA和杂交三链体的稳定Hoogsteen配对寡聚物双链体。
Nucleic Acids Res. 1999 Aug 15;27(16):3371-9. doi: 10.1093/nar/27.16.3371.
9
Hoogsteen DNA duplexes of 3'-3'- and 5'-5'-linked oligonucleotides and trip formation with RNA and DNA pyrimidine single strands: experimental and molecular modeling studies.3'-3'-和5'-5'-连接的寡核苷酸的Hoogsteen DNA双链体以及与RNA和DNA嘧啶单链形成三链体：实验和分子建模研究
Biochemistry. 1996 Dec 3;35(48):15332-9. doi: 10.1021/bi961505y.
10
Thermal stability of G-rich anti-parallel DNA triplexes upon insertion of LNA and α-L-LNA.插入锁核酸（LNA）和α-L-锁核酸（α-L-LNA）后富含鸟嘌呤的反平行DNA三链体的热稳定性
Org Biomol Chem. 2015 May 14;13(18):5115-21. doi: 10.1039/c5ob00535c.

引用本文的文献

1
An efficient alternative to DBU in the oxathiaphospholane (OTP) method for the solid phase synthesis of P-stereodefined phosphorothioate analogs.在用于固相合成P-立体定向硫代磷酸酯类似物的氧杂硫磷杂环戊烷（OTP）方法中，一种替代DBU的有效试剂。
RSC Adv. 2024 Jul 4;14(29):21174-21179. doi: 10.1039/d4ra02833c. eCollection 2024 Jun 27.
2
Review of fragmentation of synthetic single-stranded oligonucleotides by tandem mass spectrometry from 2014 to 2022.2014 年至 2022 年串联质谱法分析合成单链寡核苷酸碎片化的研究综述。
Rapid Commun Mass Spectrom. 2023 Sep 15;37(17):e9596. doi: 10.1002/rcm.9596.
3
Modified Nucleotides for Chemical and Enzymatic Synthesis of Therapeutic RNA.

本文引用的文献

1
Homopurine R-stereodefined phosphorothioate analogs of DNA with hampered Watson-Crick base pairings form Hoogsteen paired parallel duplexes with (2'-OMe)-RNAs.具有受阻 Watson-Crick 碱基配对的 DNA 的同嘌呤 R-立体限定硫代磷酸酯类似物与 (2'-OMe)-RNAs 形成 Hoogsteen 配对的平行双链体。
Org Biomol Chem. 2019 May 8;17(18):4611-4620. doi: 10.1039/c8ob03112f.
2
Revisiting sulfur H-bonds in proteins: The example of peroxiredoxin AhpE.重新审视蛋白质中的硫氢键：以过氧化物酶 AhpE 为例。
Sci Rep. 2016 Jul 29;6:30369. doi: 10.1038/srep30369.
3
New Perspectives on DNA and RNA Triplexes As Effectors of Biological Activity.
用于治疗性RNA化学合成和酶促合成的修饰核苷酸。
Curr Med Chem. 2023;30(11):1320-1347. doi: 10.2174/0929867330666221014111403.
作为生物活性效应物的DNA和RNA三链体的新视角。
PLoS Genet. 2015 Dec 23;11(12):e1005696. doi: 10.1371/journal.pgen.1005696. eCollection 2015 Dec.
4
Thermal stability and conformation of antiparallel duplexes formed by P-stereodefined phosphorothioate DNA/LNA chimeric oligomers with DNA and RNA matrices.由具有DNA和RNA基质的P-立体定向硫代磷酸酯DNA/LNA嵌合寡聚物形成的反平行双链体的热稳定性和构象。
Org Biomol Chem. 2015 Oct 21;13(39):10032-40. doi: 10.1039/c5ob01474c.
5
LNA units present in the (2'-OMe)-RNA strand stabilize parallel duplexes (2'-OMe)-RNA/[All-R(P)-PS]-DNA and parallel triplexes (2'-OMe)-RNA/[All-R(P)-PS]-DNA/RNA. An improved tool for the inhibition of reverse transcription.在（2'-O-甲基）RNA 链中存在的 LNA 单元稳定平行双链（2'-O-甲基）RNA/[All-R(P)-PS]-DNA 和平行三聚体（2'-O-甲基）RNA/[All-R(P)-PS]-DNA/RNA。一种改进的逆转录抑制工具。
Org Biomol Chem. 2015 Feb 28;13(8):2375-84. doi: 10.1039/c4ob02364a.
6
The TTSMI database: a catalog of triplex target DNA sites associated with genes and regulatory elements in the human genome.TTSMI数据库：人类基因组中与基因和调控元件相关的三链体靶DNA位点目录。
Nucleic Acids Res. 2015 Jan;43(Database issue):D110-6. doi: 10.1093/nar/gku970. Epub 2014 Oct 16.
7
Recognition of RNA duplexes by chemically modified triplex-forming oligonucleotides.化学修饰的三聚体形成寡核苷酸识别 RNA 双链。
Nucleic Acids Res. 2013 Jul;41(13):6664-73. doi: 10.1093/nar/gkt352. Epub 2013 May 8.
8
Formation of triple-helical structures by the 3'-end sequences of MALAT1 and MENβ noncoding RNAs.MALAT1 和 MENβ 非编码 RNA 3'-末端序列形成三螺旋结构。
Proc Natl Acad Sci U S A. 2012 Nov 20;109(47):19202-7. doi: 10.1073/pnas.1217338109. Epub 2012 Nov 5.
9
Phosphorothioate nucleotides and oligonucleotides - recent progress in synthesis and application.硫代磷酸酯核苷酸和寡核苷酸——合成与应用的最新进展
Chem Biodivers. 2011 Sep;8(9):1642-81. doi: 10.1002/cbdv.201100130.
10
Potential in vivo roles of nucleic acid triple-helices.核酸三螺旋的体内潜在作用。
RNA Biol. 2011 May-Jun;8(3):427-39. doi: 10.4161/rna.8.3.14999. Epub 2011 May 1.