The structural composition of components contributes to the superiority of the geoherb for "diuresis and diffusing dampness".

The purpose of this study is to explore the intrinsic reasons for the superiority of the salt-made geoherb comparing the content of various components of the salt-made geoherb . The effects of "diuresis and diffusing dampness" using salt-made from seven different origins were investigated through pharmacodynamic experiments and . The results indicated that salt-made from different origins had diuretic efficacy; this was demonstrated by the significant increase in the volume of rat urine, the concentration of Na, K, and Cl in the urine, and the significant decrease in the levels of AQP-2 in rat renal medulla and HK-2 cells. It was also revealed that the diuretic effect of salt-made from Fujian Province is stronger than those from other provinces. Moreover, the main components and their proportions in the salt-made samples were further analyzed principal component analysis. The results showed that alisol A 24-acetate, alisol B, and 23-acetyl alisol B are the main components of salt-made , and the optimal structural ratio of alisol A 24-acetate, alisol B, and 23-acetyl alisol B was found to be 5.38 : 14.34 : 11.31 optimizing the ratios of the three main components. It is worth noting that the optimal ratio of the three main components after optimization is the closest to the ratio of the three main components in salt-made from Fujian Province. This paper reveals the "mystery" of the content ratio of the main active components and its effect on the efficacy, and showed that the proportional relationship between the content of multiple components is the key to their interactions. Therefore, this method of evaluating the quality of salt-made is obviously reliable, and this study lays the foundations for quality evaluation of salt-made and other herb slices.