Li Ru, Li Zhi-Li, Chen Ya-Ping, Bu Wei-Quan, Ding Wen-Bo, Yang Bing, Wang Chun-Fei, Ma Liang, Jia Xiao-Bin, Feng Liang
School of Traditional Chinese Pharmacy, China Pharmaceutical University Nanjing 211198 P.R.China
Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Third Clinical Medical College, Nanjing University of Chinese Medicine Nanjing Jiangsu 210028 P.R. China
RSC Adv. 2020 Oct 27;10(65):39385-39395. doi: 10.1039/c9ra08469j.
The purpose of this study is to explore the intrinsic reasons for the superiority of the salt-made geoherb comparing the content of various components of the salt-made geoherb . The effects of "diuresis and diffusing dampness" using salt-made from seven different origins were investigated through pharmacodynamic experiments and . The results indicated that salt-made from different origins had diuretic efficacy; this was demonstrated by the significant increase in the volume of rat urine, the concentration of Na, K, and Cl in the urine, and the significant decrease in the levels of AQP-2 in rat renal medulla and HK-2 cells. It was also revealed that the diuretic effect of salt-made from Fujian Province is stronger than those from other provinces. Moreover, the main components and their proportions in the salt-made samples were further analyzed principal component analysis. The results showed that alisol A 24-acetate, alisol B, and 23-acetyl alisol B are the main components of salt-made , and the optimal structural ratio of alisol A 24-acetate, alisol B, and 23-acetyl alisol B was found to be 5.38 : 14.34 : 11.31 optimizing the ratios of the three main components. It is worth noting that the optimal ratio of the three main components after optimization is the closest to the ratio of the three main components in salt-made from Fujian Province. This paper reveals the "mystery" of the content ratio of the main active components and its effect on the efficacy, and showed that the proportional relationship between the content of multiple components is the key to their interactions. Therefore, this method of evaluating the quality of salt-made is obviously reliable, and this study lays the foundations for quality evaluation of salt-made and other herb slices.
本研究旨在通过比较盐制地产药材的各种成分含量，探索盐制地产药材优势的内在原因。通过药效学实验研究了七种不同产地的盐制药材的“利尿渗湿”作用。结果表明，不同产地的盐制药材具有利尿功效；大鼠尿量、尿中Na、K和Cl浓度显著增加，大鼠肾髓质和HK-2细胞中AQP-2水平显著降低，证明了这一点。还发现福建产盐制药材的利尿作用强于其他省份。此外，通过主成分分析进一步分析了盐制药材样品中的主要成分及其比例。结果表明，泽泻醇A 24-乙酸酯、泽泻醇B和23-乙酰泽泻醇B是盐制药材的主要成分，通过优化三种主要成分的比例，发现泽泻醇A 24-乙酸酯、泽泻醇B和23-乙酰泽泻醇B的最佳结构比例为5.38∶14.34∶11.31。值得注意的是，优化后三种主要成分的最佳比例最接近福建产盐制药材中三种主要成分的比例。本文揭示了主要活性成分含量比例的“奥秘”及其对药效的影响，并表明多种成分含量之间的比例关系是它们相互作用的关键。因此，这种评价盐制药材质量的方法显然是可靠的，本研究为盐制药材及其他饮片的质量评价奠定了基础。
