Degen S J, Davie E W
Ann N Y Acad Sci. 1986;485:66-72. doi: 10.1111/j.1749-6632.1986.tb34568.x.
The gene coding for human prothrombin has been isolated from two human genomic DNA libraries using a human prothrombin cDNA. Present evidence indicates that the gene is approximately 24 kb in length with about 90% of the DNA representing intervening sequence. Thirteen intervening sequences were found to interrupt the region coding for the mRNA into 14 exons. These intervening sequences vary greatly in size and contain at least 11 copies of Alu repetitive DNA. The positions where several of the intervening sequences interrupt the coding region appear to separate functional and structural domains of the protein. A similar placement of intervening sequences in genes coding for proteins homologous to prothrombin has been observed and provides additional evidence that these proteins have evolved from a common ancestor.
利用人凝血酶原cDNA，已从两个人基因组DNA文库中分离出编码人凝血酶原的基因。现有证据表明，该基因长度约为24kb，约90%的DNA为间隔序列。发现13个间隔序列将编码mRNA的区域打断成14个外显子。这些间隔序列大小差异很大，并且包含至少11个拷贝的Alu重复DNA。几个间隔序列打断编码区的位置似乎分隔了该蛋白质的功能和结构域。在编码与凝血酶原同源的蛋白质的基因中，也观察到了间隔序列的类似排列方式，这为这些蛋白质起源于共同祖先提供了额外证据。
