Li Zhishang, Xu Xiahong, Fu Yingchun, Guo Yuna, Zhang Qi, Zhang Qiaoyan, Yang Hua, Li Yanbin
College of Biosystems Engineering and Food Science, Zhejiang University 866 Yuhangtang Road Hangzhou 310058 China
State Key Lab Breeding Base for Zhejiang Sustainable Plant Pest Control, Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences Hangzhou 310021 China.
RSC Adv. 2019 Jan 2;9(2):620-625. doi: 10.1039/c8ra07804a.
Sensitive and rapid detection of aflatoxin B1 (AFB1) without using antibody or biomolecular modifications in water is achieved using a novel water-stable luminescent metal-organic framework (LMOF) termed Zr-CAU-24. The 1,2,4,5-tetrakis(4-carboxyphenyl) benzene (HTCPB)-based LMOF with high water-stability has demonstrated drastic fluorescence fading in the presence of AFB1. The detection limit for AFB1 using this porous nanomaterial reaches as low as 19.97 ppb (64 nM), which is below the applicable action level for peanut and corn products set by the FDA and among the most sensitive sensors reported for AFB1. We further investigated its response to five other mycotoxins including AFB2, AFG1, AFG2, AFM and OTA and their Stern-Volmer quenching efficiencies are significantly below that of AFB1 (138 461 M). The prepared water-stable LMOF was directly used for the detection of AFB1 in spiked walnut and almond beverages. High recovery rates (91-108%) were achieved in 5 min. We found that the quenching of HTCPB molecules towards mycotoxins was remarkably enhanced by anchoring them into the periodic framework and its mechanism was discussed. The presented method with acceptable detection limit is of potential for the development of low-cost, robust and sensitive sensors for the rapid detection of AFB1 in agricultural and food products.
使用一种名为Zr-CAU-24的新型水稳定发光金属有机框架（LMOF），无需使用抗体或生物分子修饰，就能在水中灵敏且快速地检测黄曲霉毒素B1（AFB1）。这种基于1,2,4,5-四（4-羧基苯基）苯（HTCPB）且具有高水稳定性的LMOF，在AFB1存在时表现出显著的荧光猝灭。使用这种多孔纳米材料检测AFB1的检测限低至19.97 ppb（64 nM），低于美国食品药品监督管理局（FDA）设定的花生和玉米产品适用行动水平，并且是已报道的对AFB1最灵敏的传感器之一。我们进一步研究了它对包括AFB2、AFG1、AFG2、AFM和OTA在内的其他五种霉菌毒素的响应，发现它们的斯特恩-沃尔默猝灭效率显著低于AFB1（138461 M）。所制备的水稳定LMOF被直接用于检测加标的核桃和杏仁饮料中的AFB1。在5分钟内实现了高回收率（91 - 108%）。我们发现将HTCPB分子锚定到周期性框架中可显著增强其对霉菌毒素的猝灭作用，并对其机制进行了讨论。所提出的方法具有可接受的检测限，对于开发低成本、耐用且灵敏的传感器以快速检测农产品和食品中的AFB1具有潜力。
