探索23F血清型胸苷酸转移酶Cps23FL广泛的三磷酸核苷酸和糖-1-磷酸特异性。
Exploring the broad nucleotide triphosphate and sugar-1-phosphate specificity of thymidylyltransferase Cps23FL from serotype 23F.
作者信息
Li Siqiang, Wang Hong, Jin Guoxia, Chen Zonggang, Gu Guofeng
机构信息
National Glycoengineering Research Center, Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University 72 Binhai Road Qingdao 266237 China
School of Biological and Food Processing Engineering, Huanghuai University 76 Kaiyuan Road Zhumadian 463000 China.
出版信息
RSC Adv. 2020 Aug 14;10(50):30110-30114. doi: 10.1039/d0ra05799a. eCollection 2020 Aug 10.
Glucose-1-phosphate thymidylyltransferase (Cps23FL) from serotype 23F is the initial enzyme that catalyses the thymidylyl transfer reaction in prokaryotic deoxythymidine diphosphate-l-rhamnose (dTDP-Rha) biosynthetic pathway. In this study, the broad substrate specificity of Cps23FL towards six glucose-1-phosphates and nine nucleoside triphosphates as substrates was systematically explored, eventually providing access to nineteen sugar nucleotide analogs.
来自23F血清型的葡萄糖-1-磷酸胸苷酰转移酶（Cps23FL）是原核生物二磷酸脱氧胸苷-L-鼠李糖（dTDP-Rha）生物合成途径中催化胸苷酰转移反应的起始酶。在本研究中，系统地探索了Cps23FL对六种葡萄糖-1-磷酸酯和九种核苷三磷酸作为底物的广泛底物特异性，最终获得了十九种糖核苷酸类似物。
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