Dong Jiao, Zhang Xianxin, Qu Chunxiao, Rong Xuedong, Liu Jie, Qu Yiqing
Department of Respiratory Medicine, Qilu Hospital of Shandong University Jinan Shandong 250012 China
Department of Respiratory Medicine, Shandong Provincial Chest Hospital Jinan 250013 China.
RSC Adv. 2019 Mar 12;9(15):8300-8309. doi: 10.1039/c9ra00090a.
Oligopeptides are rarely reported from Chinese herbal medicine because they are often present in very low concentrations in a complex matrix. Twenty-eight oligopeptides were recently identified by high-performance liquid chromatography and quadrupole-time-of-flight-mass spectrometry (HPLC-Q-TOF-MS) from L. (Cucurbitaceae), and a septapeptide, FHGKGHE (Phe-His-Gly-Lys-Gly-His-Glu), named MCLO-12, showed the best anticancer activity against the non-small cell lung cancer A549 cell line , with an IC value of 21.4 ± 2.21 mM. The anti-proliferative activity assay results showed that MCLO-12 induced apoptosis of A549 cells in a concentration-dependent manner. Treatment of the cells with MCLO-12 (10.7-42.8 mM mL) caused strong intracellular reactive oxygen species (ROS) up-regulating activities and activated caspase expression. MCLO-12 also suppressed the Trx system and subsequently activated a number of Trx-dependent pathways, including the ASK1, MAPK-p38 and JNK pathways. Thus, our research provides a good reference point for anti-NSCLC research into oligopeptides.
由于寡肽在复杂基质中的含量通常非常低，因此从中药中提取寡肽的报道很少。最近通过高效液相色谱和四极杆飞行时间质谱（HPLC-Q-TOF-MS）从罗汉果（葫芦科）中鉴定出28种寡肽，其中一种七肽FHGKGHE（苯丙氨酸-组氨酸-甘氨酸-赖氨酸-甘氨酸-组氨酸-谷氨酸），命名为MCLO-12，对非小细胞肺癌A549细胞系显示出最佳的抗癌活性，IC值为21.4±2.21 mM。抗增殖活性测定结果表明，MCLO-12以浓度依赖性方式诱导A549细胞凋亡。用MCLO-12（10.7-42.8 mM/mL）处理细胞会引起强烈的细胞内活性氧（ROS）上调活性并激活半胱天冬酶表达。MCLO-12还抑制了硫氧还蛋白系统，随后激活了许多硫氧还蛋白依赖性途径，包括ASK1、MAPK-p38和JNK途径。因此，我们的研究为寡肽抗非小细胞肺癌的研究提供了很好的参考依据。
