Yang Yi-Xuan, Fang Yan-Zhao, Tian Jing-Xuan, Xiao Qiang, Kong Xiang-Juan
Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University Nanchang 330013 P. R. China
RSC Adv. 2020 Jul 27;10(47):28164-28170. doi: 10.1039/d0ra02031a.
Herein, a novel fluorescence nanosensor using intrinsic fluorescent polydopamine nanoparticles (PDA NPs) as an effective signal reporter has been constructed for the simple, rapid and sequential detection of mercury ions (Hg) and l-ascorbic acid (AA) based on a coordination effect and redox reaction. The fluorescence of the PDA NPs could be specifically quenched by Hg through intense coordination effects between the Hg and the groups (catechol, amine, ketone and imine) on the surface of the PDA NPs. However, when AA and Hg coexisted in solution, the fluorescence of the PDA NPs pronouncedly recovered the redox reaction of Hg, with it being reduced to Hg by AA. The fluorescence quenching mechanism of Hg towards the PDA NPs and the redox reaction between Hg and AA were also fully investigated. The nanosensor exhibited high sensitivity and desirable selectivity for Hg and AA detection. Moreover, the strategy was successfully explored in real samples (tap water, lake water and human serum samples) with satisfactory recoveries. The developed nanosensor provides new sights and good inspiration for Hg and AA detection under real conditions.
在此，基于配位效应和氧化还原反应，构建了一种新型荧光纳米传感器，该传感器使用本征荧光聚多巴胺纳米颗粒（PDA NPs）作为有效的信号报告器，用于简单、快速且顺序检测汞离子（Hg）和L-抗坏血酸（AA）。Hg与PDA NPs表面的基团（儿茶酚、胺、酮和亚胺）之间的强烈配位作用可使PDA NPs的荧光特异性猝灭。然而，当AA和Hg共存于溶液中时，由于Hg被AA还原为Hg，PDA NPs的荧光显著恢复。同时，还对Hg对PDA NPs的荧光猝灭机制以及Hg与AA之间的氧化还原反应进行了充分研究。该纳米传感器对Hg和AA的检测具有高灵敏度和良好的选择性。此外，该策略在实际样品（自来水、湖水和人血清样品）中得到成功应用，回收率令人满意。所开发的纳米传感器为实际条件下Hg和AA的检测提供了新的视角和良好的启发。
