Huang Lu, Zhang Sheng, Zhou Jun, Li Xiangzhou
School of Materials Science and Engineering, Central South University of Forestry and Technology 498 South Shaoshan Ave Changsha 410004 Hunan China.
National Engineering Laboratory of Southern Forestry Ecological Application Technology Changsha 410004 China.
RSC Adv. 2019 Jan 18;9(5):2572-2580. doi: 10.1039/c8ra08364a.
To investigate the effects of resveratrol on the drug resistance of 5-FU in the colon cancer chemotherapy, an MTT assay was used to detect the effects of 5-FU and resveratrol combined with 5-FU on the proliferation of the LoVo and SW480 cell lines. Flow cytometry was used to detect the effect of 5-FU combined with resveratrol on the survival rate of the LoVo and SW480 cells. A western blot was used to detect the expression levels of the proteins associated with colon cancer. After flow sorting, the percentage of the SW480 and the LoVo cell line CD133 was 97.5% and 95.8%, respectively. The cells cultured showed more rapid cell proliferation and differentiation. The MTT assay showed that as compared with the survival rate of the blank group LoVo and CD133 LoVo cells, the survival rate of the cells containing the 5-FU group was lower ( < 0.05). When 5-FU was used in combination with different concentrations of resveratrol, the abovementioned phenomenon was more prominent. The sorted colon cancer cells have dry stem cells, and the sorted CD133 cells are more resistant to drugs; the combination of resveratrol and 5-FU has the best effect on the colon cancer cells. Preliminary studies on the mechanism of action of the drug show that a combination of 5-FU and resveratrol regulates apoptosis in CD133 colon cancer stem cells by regulating the gene; however, more complex mechanisms may also be involved.
为研究白藜芦醇对结肠癌化疗中5-氟尿嘧啶耐药性的影响，采用MTT法检测5-氟尿嘧啶及白藜芦醇联合5-氟尿嘧啶对LoVo和SW480细胞系增殖的影响。采用流式细胞术检测5-氟尿嘧啶联合白藜芦醇对LoVo和SW480细胞存活率的影响。采用蛋白质印迹法检测与结肠癌相关的蛋白质表达水平。流式分选后，SW480和LoVo细胞系CD133的百分比分别为97.5%和95.8%。培养的细胞显示出更快的细胞增殖和分化。MTT法显示，与空白组LoVo和CD133 LoVo细胞的存活率相比，含5-氟尿嘧啶组细胞的存活率较低（<0.05）。当5-氟尿嘧啶与不同浓度的白藜芦醇联合使用时，上述现象更为明显。分选后的结肠癌细胞具有干性干细胞，分选后的CD133细胞对药物更具抗性；白藜芦醇与5-氟尿嘧啶联合使用对结肠癌细胞的效果最佳。对该药物作用机制的初步研究表明，5-氟尿嘧啶与白藜芦醇联合通过调节 基因来调节CD133结肠癌干细胞中的细胞凋亡；然而，可能还涉及更复杂的机制。
