Gao Jin, Lan Tinxi, Zong Xumin, Shi Gensheng, He Shuqing, Cui Fengmei, Tu Yu
State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Soochow University, Suzhou, China.
Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Suzhou, China.
Dose Response. 2022 Apr 29;20(2):15593258221088745. doi: 10.1177/15593258221088745. eCollection 2022 Apr-Jun.
The health of radiation workers has always been our focus. Epidemiological investigation shows that long-term exposure to low-dose ionizing radiation can affect human health, especially cancer and cardiovascular disease, and there are many studies on it. However, up to now, there have been few reports on the research of blood and biological samples from radiation workers. In this study, radiation workers and healthy control groups were strictly screened, and the transcriptome of mRNA and circRNA was sequenced by extracting their peripheral venous blood. At the same time, appropriate data sets were selected in the GEO database for bioinformatics analysis, and circRNA-miRNA-mRNA network was constructed. We identified 9 different circular ribonucleic acids, 3 tiny ribonucleic acids, and 2 central genes (NOD 2 and IRF 7). These differentially expressed genes and non-coding RNA are closely related to ionizing radiation damage, and play an important role as biological markers. In conclusion, this study may provide new insights into the role of the circRNA-miRNA-mRNA regulatory network in the health of radiation workers, and provides a new strategy for the future study of radiation biology.
辐射工作人员的健康一直是我们关注的焦点。流行病学调查表明，长期暴露于低剂量电离辐射会影响人体健康，尤其是癌症和心血管疾病，对此已有许多研究。然而，截至目前，关于辐射工作人员血液和生物样本的研究报道较少。在本研究中，对辐射工作人员和健康对照组进行了严格筛选，通过提取他们的外周静脉血对mRNA和circRNA的转录组进行测序。同时，在GEO数据库中选择合适的数据集进行生物信息学分析，并构建circRNA-miRNA-mRNA网络。我们鉴定出9种不同的环状核糖核酸、3种微小核糖核酸和2个核心基因（NOD 2和IRF 7）。这些差异表达的基因和非编码RNA与电离辐射损伤密切相关，作为生物标志物发挥着重要作用。总之，本研究可能为circRNA-miRNA-mRNA调控网络在辐射工作人员健康中的作用提供新的见解，并为未来辐射生物学的研究提供新策略。
