Department of Geriatrics, General Hospital of Eastern Theater Command, Nanjing, China.
Department of Geriatrics, The First People's Hospital of Changzhou, Changzhou, China.
Immunopharmacol Immunotoxicol. 2022 Oct;44(5):656-662. doi: 10.1080/08923973.2022.2074448. Epub 2022 Jun 6.
Ischemic stroke is the most common type of acute cerebrovascular disease. Carnosol is a polyphenol compound extracted from rosemary. This study aimed to explore the effects of carnosol on the oxygen-glucose deprivation (OGD) treated BV2 microglia cells. MTT and EdU assays were used to detect the cell viability and proliferation. Flow cytometry was conducted to measure the apoptosis rates. Additionally, the protein expression was determined by western blot. The inflammatory factors and antioxidant indexes were detected by corresponding kits. Carnosol promoted the proliferation and inhibited the apoptosis of the OGD treated BV2 cells. What's more, the protein expressions of PCNA and Bcl-2 were up-regulated, the Bax expression was down-regulated after carnosol treatment. In addition, carnosol decreased the levels of MDA, LPO, TNF-α, IL-1β and IL-6, and increased the levels of GSH, SOD, IL-4 and IL-10 in the OGD treated BV2 cells. Furthermore, the PI3K/AKT/mTOR signaling pathway was activated after carnosol treatment and inhibition of the PI3K/AKT/mTOR signaling pathway reversed the carnosol effects. Carnosol promotes the proliferation, inhibits the apoptosis, relieves the oxidative damage and inflammation of OGD treated cells through regulating the PI3K/AKT/mTOR signaling pathway.
缺血性脑卒中是最常见的急性脑血管病。迷迭香酚是从迷迭香中提取的一种多酚化合物。本研究旨在探讨迷迭香酚对氧葡萄糖剥夺（OGD）处理的 BV2 小胶质细胞的影响。MTT 和 EdU 检测分别用于检测细胞活力和增殖。流式细胞术用于测量细胞凋亡率。此外，通过 Western blot 测定蛋白质表达。用相应的试剂盒检测炎症因子和抗氧化指标。迷迭香酚促进 OGD 处理的 BV2 细胞的增殖并抑制其凋亡。更重要的是，迷迭香酚处理后 PCNA 和 Bcl-2 的蛋白表达上调，Bax 的表达下调。此外，迷迭香酚降低了 OGD 处理的 BV2 细胞中 MDA、LPO、TNF-α、IL-1β 和 IL-6 的水平，增加了 GSH、SOD、IL-4 和 IL-10 的水平。此外，迷迭香酚处理后激活了 PI3K/AKT/mTOR 信号通路，而 PI3K/AKT/mTOR 信号通路的抑制逆转了迷迭香酚的作用。迷迭香酚通过调节 PI3K/AKT/mTOR 信号通路促进 OGD 处理细胞的增殖，抑制其凋亡，减轻氧化损伤和炎症。
