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正常组织和恶性组织中一种分子量为34,000的人类上皮特异性表面糖蛋白表达的免疫组织化学研究。

Immunohistochemical study of the expression of a Mr 34,000 human epithelium-specific surface glycoprotein in normal and malignant tissues.

作者信息

Momburg F, Moldenhauer G, Hämmerling G J, Möller P

出版信息

Cancer Res. 1987 Jun 1;47(11):2883-91.

PMID:3552208
Abstract

Monoclonal antibody HEA125 was used to study the tissue distribution of an epithelial cell surface glycoprotein of Mr 34,000 (Egp34). A large panel of normal and neoplastic tissues was examined for immunoreactivity with HEA125 by means of a sensitive immunoperoxidase technique. HEA125 labeled most epithelial cell types throughout the body but did not label any nonepithelial tissue. Major exceptions were epidermal keratinocytes, gastric parietal cells, hepatocytes, thymic cortical epithelial, and myoepithelial cells. Normal mesothelial cells were unreactive. In normal glandular epithelia and tubular adenocarcinomas exclusively the basolateral cell membranes were stained. HEA125 intensely reacted with all tested carcinoma specimens derived from colorectum, stomach, pancreas, liver, lung, mammary gland, ovary, thyroid, kidney, urinary bladder, and prostate including a number of anaplastic, diffusely infiltrating carcinomas. Metastatic lesions of these tumors were consistently positive. Generally, the staining of tumor cells was very homogeneous. The majority of squamous cell carcinomas were less strongly labeled than adenocarcinomas; keratinizing areas of the tumor masses were negative. Germ cell tumors and mesotheliomas of epithelioid type focally expressed the antigen. Egp34 was found to be absent from sarcomas, lymphomas, melanomas, and neurogenic tumors. Hence, HEA125 is a useful reagent for the distinction of carcinomas from nonepithelial neoplasms, even at very low degrees of histological differentiation. Furthermore, HEA125 allows the immunohistochemical detection of micrometastases originating from carcinomas. The antigen is detectable in formalin-fixed paraffin sections.

摘要

单克隆抗体HEA125用于研究一种分子量为34,000的上皮细胞表面糖蛋白(Egp34)的组织分布。通过灵敏的免疫过氧化物酶技术,检测了一大组正常组织和肿瘤组织与HEA125的免疫反应性。HEA125标记了全身大多数上皮细胞类型,但未标记任何非上皮组织。主要的例外是表皮角质形成细胞、胃壁细胞、肝细胞、胸腺皮质上皮细胞和肌上皮细胞。正常间皮细胞无反应。在正常腺上皮和管状腺癌中,仅基底外侧细胞膜被染色。HEA125与所有检测的源自结肠、胃、胰腺、肝、肺、乳腺、卵巢、甲状腺、肾、膀胱和前列腺的癌标本强烈反应,包括一些间变性、弥漫浸润性癌。这些肿瘤的转移灶始终呈阳性。一般来说,肿瘤细胞的染色非常均匀。大多数鳞状细胞癌的标记强度低于腺癌;肿瘤块的角化区域为阴性。生殖细胞肿瘤和上皮样间皮瘤局部表达该抗原。发现肉瘤、淋巴瘤、黑色素瘤和神经源性肿瘤中不存在Egp34。因此,HEA125是区分癌与非上皮性肿瘤的有用试剂,即使在组织学分化程度很低的情况下也是如此。此外,HEA125可用于免疫组化检测源自癌的微转移。该抗原在福尔马林固定石蜡切片中可检测到。

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