Study on the role of heat shock protein 90 () gene in chicken preadipocytes proliferation and differentiation.

In this study, we examined the effects of Heat Shock Protein 90 () on adipocyte proliferation and differentiation in chickens. To achieve this, we constructed RNA interference (RNAi) vectors to target and transfected the vectors into primary adipocytes. After transfection, oil red O staining was performed to determine the status of triglyceride accumulation in the cells, whereas the CCK-8 cell kit and 5-Ethynyl-2'-Deoxyuridine (EdU) assays were used to determine cell proliferation. Thereafter, the mRNA and protein expression levels of , , and were determined, and the results showed that after the interference of , the mRNA and protein expression levels of in the chicken adipocytes decreased significantly compared to the control and blank groups (). The decreased mRNA and protein expression of , , and was related to adipocyte differentiation (). However, interference had no effect on adipocyte proliferation (). Taken together, the results of this study showed that influenced the expression of and adipose-differentiation-related genes, thereby regulating triglyceride accumulation and adipocyte differentiation in chickens.