解析 D1 抑制角膜炎中的角膜炎症。
Resolvin D1 Inhibits Corneal Inflammation in Keratitis.
机构信息
Department of Ophthalmology, Pusan National University School of Medicine, Yangsan, South Korea.
Department of Ophthalmology, Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan, South Korea.
出版信息
Ocul Immunol Inflamm. 2023 Jul;31(5):927-934. doi: 10.1080/09273948.2022.2070504. Epub 2022 May 6.
PURPOSE
To investigate the role of lipid mediator, resolvin D1 (RvD1), in corneal inflammation.
METHODS
The anti-inflammatory effect of RvD1 on stimulated human corneal epithelial cells (HCECs) was assessed. C57BL/6 mice corneas were abraded and treated with RvD1 after stimulation with . Cytokine levels in the corneas, cervical drainage lymph nodes (DLNs), and spleens were measured. Anterior segment photography and optical coherence tomography quantified the changes in corneal thickness and haziness. Neutrophil infiltration in the cornea was examined by haematoxylin and eosin (H&E) staining and immunohistochemistry.
RESULTS
RvD1 significantly inhibited cytokine production in HCECs and mouse corneas, cervical DLNs, and spleens while stimulating interleukin-10 (IL-10) production. Corneal opacity development, thickening, and neutrophil infiltration significantly reduced in response to RvD1 stimulation in the -infected mice corneas.
CONCLUSION
RvD1 inhibited -induced corneal inflammation. These results potentiate RvD1 as an anti-inflammatory therapy for patients with corneal inflammation induced by bacterial keratitis.
目的
研究脂质介质（RvD1）在角膜炎症中的作用。
方法
评估 RvD1 对刺激的人角膜上皮细胞（HCEC）的抗炎作用。在刺激后，用 RvD1 处理 C57BL/6 小鼠的角膜。测量角膜、颈引流淋巴结（DLN）和脾脏中的细胞因子水平。眼前段摄影和光学相干断层扫描定量评估角膜厚度和混浊度的变化。通过苏木精和伊红（H&E）染色和免疫组织化学检查角膜中的中性粒细胞浸润。
结果
RvD1 显著抑制 HCEC 以及小鼠角膜、颈 DLN 和脾脏中的细胞因子产生，同时刺激白细胞介素-10（IL-10）产生。在 -感染的小鼠角膜中，RvD1 刺激后角膜混浊度发展、增厚和中性粒细胞浸润显著减少。
结论
RvD1 抑制了 -诱导的角膜炎症。这些结果表明 RvD1 可作为治疗由细菌性角膜炎引起的角膜炎症的抗炎治疗药物。
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