尝试通过使用转染基因作为探针来检测神经视网膜向晶状体转分化过程中的决定状态。
An attempt to assay the state of determination by using transfected genes as probes in transdifferentiation of neural retina into lens.
作者信息
Kondoh H, Ueda Y, Hayashi S, Okazaki K, Yasuda K, Okada T S
出版信息
Cell Differ. 1987 Mar;20(2-3):203-7. doi: 10.1016/0045-6039(87)90435-0.
Hybrid genes coding for chloramphenicol acetyltransferase (CAT) with a non-specific retroviral, lens-specific delta-crystallin or lens-specific alpha-crystallin promoters were constructed to transfect the transdifferentiating (lentoidogenic) and non-transdifferentiating (non-lentoidogenic) cultures of chicken embryonic neural retina for assaying the state of determination towards lens differentiation. The expression occurred only when CAT genes with lens-specific promoters were transfected to the cultures maintained in the conditions permissive to lentoidogenesis. The expression of these exogenous, lens-specific CAT genes began at stages of culturing that were earlier than the expression of endogenous crystallin. Presumably, there are two steps in the transdifferentiation of neural retina into lens; acquisition of capacity to express crystallin genes and derepression of the endogenous crystallin genes.
构建了编码氯霉素乙酰转移酶（CAT）的杂种基因，这些基因带有非特异性逆转录病毒启动子、晶状体特异性δ-晶状体蛋白启动子或晶状体特异性α-晶状体蛋白启动子，用于转染鸡胚神经视网膜的转分化（形成类晶状体）和非转分化（非类晶状体形成）培养物，以检测晶状体分化的决定状态。只有当带有晶状体特异性启动子的CAT基因被转染到在允许类晶状体形成的条件下维持的培养物中时，才会发生表达。这些外源的、晶状体特异性CAT基因的表达开始于培养阶段，早于内源性晶状体蛋白的表达。据推测，神经视网膜向晶状体的转分化有两个步骤：获得表达晶状体蛋白基因的能力和内源性晶状体蛋白基因的去抑制。
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