Department of Ophthalmology, Eye, Ear, Nose, and Throat Hospital of Fudan University, Shanghai 200000, Shanghai, China.
Laboratory of Myopia, NHC Key Laboratory of Myopia, Fudan University, Chinese Academy of Medical Sciences, Shanghai 200000, Shanghai, China.
Aging (Albany NY). 2022 May 6;14(9):4014-4035. doi: 10.18632/aging.204065.
Runt-related transcription factors (RUNX) are involved in numerous fundamental biological processes and play crucial parts in tumorigenesis and metastasis both directly and indirectly. However, the pan-cancer evidence of the gene family is not available.
In this study, we analyzed the potential association between gene family expression and patient's prognosis, immune cell infiltration, drug response, and genetic mutation data across different types of tumors using based on The Cancer Genome Atlas, Gene Expression Omnibus, and Oncomine database.
The results showed that the expression of the gene family varied among different cancer types, revealing its heterogeneity in cancers and that expression of RUNX2 was lower than that of RUNX1 and RUNX3 across all cancer types. gene family gene expression was related to prognosis in several cancers. Furthermore, our study revealed a clear association between gene family expression and ESTIMATE score, RNA stemness, and DNA stemness scores. Compared with RUNX1 and RUNX2, RUNX3 showed relatively low levels of genetic alterations. gene family genes had clear associations with immune infiltrate subtypes, and their expression was positively related to immune checkpoint genes and drug sensitivity in most cases. Two immunotherapy cohorts confirm that the expression of was correlated with the clinical response of immunotherapy.
These findings will help to elucidate the potential oncogenic roles of gene family genes in different types of cancer and it can function as a prognostic marker in various malignant tumors.
runt 相关转录因子（RUNX）参与了许多基本的生物学过程，并直接或间接地在肿瘤发生和转移中发挥着关键作用。然而，RUNX 基因家族在泛癌症中的证据尚不可用。
在这项研究中，我们使用基于 The Cancer Genome Atlas、Gene Expression Omnibus 和 Oncomine 数据库，分析了不同类型肿瘤中 RUNX 基因家族表达与患者预后、免疫细胞浸润、药物反应和基因突变数据之间的潜在关联。
结果表明，RUNX 基因家族的表达在不同癌症类型之间存在差异，揭示了其在癌症中的异质性，并且在所有癌症类型中，RUNX2 的表达均低于 RUNX1 和 RUNX3。RUNX 基因家族基因表达与几种癌症的预后相关。此外，我们的研究还揭示了 RUNX 基因家族表达与 ESTIMATE 评分、RNA 干性和 DNA 干性评分之间的明确关联。与 RUNX1 和 RUNX2 相比，RUNX3 表现出相对较低的遗传改变水平。RUNX 基因家族基因与免疫浸润亚型有明显的关联，其表达在大多数情况下与免疫检查点基因和药物敏感性呈正相关。两个免疫治疗队列证实，RUNX 基因家族的表达与免疫治疗的临床反应相关。
这些发现将有助于阐明 RUNX 基因家族基因在不同类型癌症中的潜在致癌作用，并可作为各种恶性肿瘤的预后标志物。
