Reference Laboratory, Autobio Diagnostics Co., Ltd, Zhengzhou, Henan, 450016, China.
Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, Jiangsu, 226001, China.
Talanta. 2022 Aug 15;246:123494. doi: 10.1016/j.talanta.2022.123494. Epub 2022 Apr 20.
The rigorous and reasonable evaluation of uncertainty is crucial for the reliability of the results of a reference measurement procedure. A new uncertainty budget is proposed for uncertainty evaluation of a reference measurement procedure for total thyroxine in human serum based on isotope dilution liquid chromatography-mass spectrometry. In this work, a measurement model for uncertainty evaluation of triple isotope dilution-mass spectrometry was established. The propagation of uncertainties assigned to various input quantities was expressed in strict accordance with the measurement model. A detailed description of the uncertainty evaluation process for the measurement result of a specific serum is given, including the estimate of the input quantities and the determination of the combined standard uncertainty. The expanded uncertainty of the specific human serum (at coverage probability of 95%) was 4.8 nmol/L at the concentration level of 113.6 nmol/L.
对不确定度进行严格而合理的评估对于参考测量程序结果的可靠性至关重要。本文提出了一种新的不确定度预算，用于评估基于同位素稀释液相色谱-质谱法的人血清总甲状腺素参考测量程序的不确定度。在这项工作中，建立了用于三重同位素稀释-质谱法不确定度评估的测量模型。按照测量模型严格表达了分配给各种输入量的不确定度的传播。详细描述了特定血清测量结果的不确定度评估过程，包括输入量的估计和合并标准不确定度的确定。在 113.6 nmol/L 浓度水平下，特定人血清（置信概率为 95%）的扩展不确定度为 4.8 nmol/L。
