Department of Clinical Laboratory, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China.
Department of Clinical Laboratory, Shanghai University of Traditional Chinese Medical Attached Shuguang Hospital, Shanghai, China.
Int J Infect Dis. 2022 Aug;121:47-54. doi: 10.1016/j.ijid.2022.04.061. Epub 2022 May 4.
To evaluate the performance of nucleotide matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) in predicting the drug resistance of Mycobacterium tuberculosis.
A total of 115 rifampin-resistant and 53 rifampin-susceptible tuberculosis (TB) clinical isolates were randomly selected from TB strains stored at -80℃ in the clinical laboratory of Shanghai Pulmonary Hospital. Nucleotide MALDI-TOF-MS was performed to predict the drug resistance using phenotypic susceptibility as the gold standard.
The overall assay sensitivities and specificities of nucleotide MALDI-TOF-MS were 92.2% and 100.0% for rifampin, 90.9% and 98.6% for isoniazid, 71.4% and 81.2% for ethambutol, 85.1% and 93.1% for streptomycin, 94.0% and 100.0% for amikacin, 77.8% and 99.3% for kanamycin, 75.0% and 93.3% for ofloxacin, and 75.0% and 93.3% for moxifloxacin. The concordances between nucleotide MALDI-TOF-MS antimicrobial susceptibility testing (AST) and phenotypic AST were 94.6% (rifampin), 90.1% (isoniazid), 79.2% (ethambutol), 89.9% (streptomycin), 99.4% (amikacin), 97.0% (kanamycin), 88.1% (ofloxacin), and 88.0% (moxifloxacin).
Nucleotide MALDI-TOF-MS could be a promising tool for rapid detection of Mycobacterium tuberculosis drug sensitivity to rifampin, isoniazid, ethambutol, streptomycin, amikacin, kanamycin, ofloxacin, and moxifloxacin.
评估核苷酸基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）在预测结核分枝杆菌耐药性方面的性能。
从上海肺科医院临床实验室储存的-80℃下的结核分枝杆菌菌株中随机选择 115 株利福平耐药和 53 株利福平敏感的结核（TB）临床分离株。采用表型药敏试验作为金标准，用核苷酸 MALDI-TOF-MS 预测耐药性。
核苷酸 MALDI-TOF-MS 对利福平、异烟肼、乙胺丁醇、链霉素、阿米卡星、卡那霉素、氧氟沙星和莫西沙星的总检测灵敏度和特异性分别为 92.2%和 100.0%、90.9%和 98.6%、71.4%和 81.2%、85.1%和 93.1%、94.0%和 100.0%、77.8%和 99.3%、75.0%和 93.3%、75.0%和 93.3%。核苷酸 MALDI-TOF-MS 药敏试验（AST）与表型 AST 的一致性分别为 94.6%（利福平）、90.1%（异烟肼）、79.2%（乙胺丁醇）、89.9%（链霉素）、99.4%（阿米卡星）、97.0%（卡那霉素）、88.1%（氧氟沙星）和 88.0%（莫西沙星）。
核苷酸 MALDI-TOF-MS 可能成为快速检测结核分枝杆菌对利福平、异烟肼、乙胺丁醇、链霉素、阿米卡星、卡那霉素、氧氟沙星和莫西沙星敏感性的有前途的工具。
