Effect of (D-Trp6, Pro9-NEt)-GnRH on the function and ultrastructure of pituitary and testis in male rats.

Groups of ten adult male Wistar rats were treated chronically with GnRH agonist for different durations. The responses of pituitary and testis to GnRH agonist were evaluated by serum and testicular testosterone, serum and pituitary LH, and testicular LH receptors. Testicular testosterone and LH receptors were decreased very significantly under our experimental regimen, irrespective of the duration of the treatment Serum testosterone levels were reduced after 10 and 15 injections, but only rats receiving 10 injections showed a significant increase in serum LH levels and decrease of pituitary LH content. The type II gonadotropes of the pars distalis of pituitary were increased in number but showed a higher degree of dilated smooth endoplasmic reticulum with few secretory granules. Many large lipid droplets appeared in the cytoplasms of the Leydig cells. Degenerative changes were observed in most seminiferous tubules following GnRH agonist treatment. Obvious abnormal acrosome and nuclear caps of some spermatids were also observed. The functional changes caused by GnRH agonist coincided with their ultrastructural appearance.