Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
Arch Microbiol. 2022 May 6;204(6):303. doi: 10.1007/s00203-022-02918-6.
Biocompatible and non-toxic properties of chitosan make it a candidate with excellent application prospects in developing wound dressing conjugate compounds. Six different chitosan-based nanohybrid membranes were evaluated against multidrug-resistant bacterial isolates. Different combinations of chitosan, ciprofloxacin (CIP), biofunctionalized montmorillonite (MMT), and montmorillonite with sulfate chains (SMMT) were provided, and their antibacterial activity was assessed using the colony count method. Totally, 27 drug-resistant isolates, including 6x methicillin-resistant Staphylococcus aureus, 7 vancomycin-resistant Enterococcus faecalis, 4 Acinetobacter baumannii, and 10 Pseudomonas aeruginosa isolates were identified from burn wound infections. Chitosan and montmorillonite did not show significant antibacterial effect (p > 0.05), but chitosan/SMMT/CIP was the most effective nanocomposite (p < 0.01). Chitosan-based nanocomposites with ciprofloxacin could effectively reduce the susceptibility of drug-resistant bacterial isolates. Bacterial targeting using nanosystems provides an opportunity for effective antibiotic treatment by improving antibacterial efficacy.
壳聚糖具有良好的生物相容性和低毒性，有望成为开发伤口敷料结合物的候选材料。本研究评估了 6 种不同的壳聚糖基纳米杂化膜对多药耐药菌分离株的作用。提供了不同组合的壳聚糖、环丙沙星（CIP）、生物功能化蒙脱石（MMT）和带硫酸链的蒙脱石（SMMT），并使用菌落计数法评估其抗菌活性。总共从烧伤感染中鉴定出 27 株耐药分离株，包括 6 株耐甲氧西林金黄色葡萄球菌、7 株耐万古霉素粪肠球菌、4 株鲍曼不动杆菌和 10 株铜绿假单胞菌。壳聚糖和蒙脱石没有表现出明显的抗菌作用（p＞0.05），但壳聚糖/SMMT/CIP 是最有效的纳米复合材料（p＜0.01）。载有环丙沙星的壳聚糖基纳米复合材料能有效降低耐药菌分离株的敏感性。纳米系统靶向细菌为提高抗菌效果提供了有效的抗生素治疗机会。
