长非编码 RNA TFAP2A-AS1 在口腔鳞状细胞癌中发挥重要作用：研究包括生物信息学分析和实验。
Long non-coding RNA TFAP2A-AS1 plays an important role in oral squamous cell carcinoma: research includes bioinformatics analysis and experiments.
机构信息
Department of Stomatology, The Fourth Hospital of Hebei Medical University, No. 12 Jiankang Road, Shijiazhuang, 050000, Hebei Province, China.
Department of Stomatology, Zhao County People's Hospital, No. 1 Yongtong Road, Shijiazhuang, 050000, Hebei Province, China.
出版信息
BMC Oral Health. 2022 May 6;22(1):160. doi: 10.1186/s12903-022-02203-4.
BACKGROUND
Oral squamous cell carcinoma (OSCC) is the most common neck and head malignancies, and the prognosis is not good. Studies shown that the long non-coding RNA (lncRNA) TFAP2A-AS1 is involved in the progression of multiple cancers. However, the role of lncRNA TFAP2A-AS1 in OSCC remains unclear. We aimed to explore the functions and expression in OSCC.
METHODS
The lncRNA profiles for OSCC patients were acquired from the TCGA. Based on these data, the data mining of TFAP2A-AS1 in patients with OSCC were performed. The functions of TFAP2A-AS1 were determined by bioinformatics analysis. The expression and roles in cell growth were tested by RT-qPCR and MTS assay. Cell invasion and migration were tested by wound healing and transwell assays.
RESULTS
The consequences displayed that TFAP2A-AS1 was upregulated in the TCGA datasets. The expression of TFAP2A-AS1 was higher in OSCC samples. Bioinformatics analysis shown that TFAP2A-AS1 might be associated with the P53 signaling pathway. Cell culture experiments indicated that deficiency of TFAP2A-AS1 inhibited cell growth, invasion, and migration, and overexpression of it could opposite results in SCC-25 cells.
CONCLUSION
The results suggested that TFAP2A-AS1 was overexpressed in OSCC cells, which could facilitate OSCC cell proliferation, migration, and invasion.
背景
口腔鳞状细胞癌（OSCC）是最常见的头颈部恶性肿瘤，预后不佳。研究表明，长链非编码 RNA（lncRNA）TFAP2A-AS1 参与多种癌症的进展。然而，lncRNA TFAP2A-AS1 在 OSCC 中的作用尚不清楚。我们旨在探讨其在 OSCC 中的功能和表达。
方法
从 TCGA 中获取 OSCC 患者的 lncRNA 图谱。基于这些数据，对 OSCC 患者的 TFAP2A-AS1 进行了数据挖掘。通过生物信息学分析确定 TFAP2A-AS1 的功能。通过 RT-qPCR 和 MTS 测定法检测其表达和在细胞生长中的作用。通过划痕愈合和 Transwell 测定法检测细胞侵袭和迁移。
结果
结果显示，TFAP2A-AS1 在 TCGA 数据集中上调。OSCC 样本中的 TFAP2A-AS1 表达水平较高。生物信息学分析表明，TFAP2A-AS1 可能与 P53 信号通路有关。细胞培养实验表明，TFAP2A-AS1 缺失抑制 SCC-25 细胞的生长、侵袭和迁移，而过表达则可产生相反的结果。
结论
结果表明，TFAP2A-AS1 在 OSCC 细胞中过表达，可促进 OSCC 细胞的增殖、迁移和侵袭。
Zotero 插件