School of Energy & Environmental Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing 100083, China; School of Environment, Beijing Normal University, No. 19, Xinjiekouwai St, Haidian District, Beijing 100875, China.
School of Environment, Beijing Normal University, No. 19, Xinjiekouwai St, Haidian District, Beijing 100875, China.
J Hazard Mater. 2022 Aug 15;436:129045. doi: 10.1016/j.jhazmat.2022.129045. Epub 2022 Apr 30.
In this study, the metal(loid) fractions in two alkaline iron tailings areas with similar physico-chemical properties and the enrichment ability of dominant plants in these areas were investigated. Additionally, high-throughput sequencing and metagenome analysis were used to examine the rhizosphere microbial community structures and their strategies and potential for carbon fixation, nitrogen metabolism, and metal(loid) resistance in mining areas. Results showed that Salsola collina, Setaria viridis, and Xanthium sibiricum have strong enrichment capacity for As, and the maximum transport factor for Mn can reach 4.01. The richness and diversity of bacteria were the highest in rhizosphere tailings, and the dominant phyla were Proteobacteria, Actinobacteria, Ascomycota, and Thaumarchaeota. The key taxa present in rhizosphere tailings were generally metal(loid) resistant, especially Sphingomonas, Pseudomonas, Nocardioides, and Microbacterium. The reductive citrate cycle was the main carbon fixation pathway of microorganisms in tailings. Rhizosphere microorganisms have evolved a series of survival strategies and can adapt to oligotrophic and metal(loid) polluted mining environments. The results of this study provide a basis for the potential application of plant-microbial in situ remediation of alkaline tailings.
在这项研究中，研究了两个具有相似物理化学性质的碱性铁尾矿区的金属（类）形态以及这些地区优势植物的富集能力。此外，还使用高通量测序和宏基因组分析来研究矿区根际微生物群落结构及其固碳、氮代谢和金属（类）抗性的策略和潜力。结果表明，滨藜、狗尾草和苍耳对砷具有很强的富集能力，锰的最大迁移因子可达 4.01。根际尾矿中的细菌丰富度和多样性最高，优势门为变形菌门、放线菌门、子囊菌门和泉古菌门。根际尾矿中存在的关键分类群通常具有抗金属（类）性，特别是节杆菌属、假单胞菌属、诺卡氏菌属和微杆菌属。还原性柠檬酸循环是微生物在尾矿中固定碳的主要途径。根际微生物已经进化出一系列生存策略，可以适应贫营养和金属（类）污染的采矿环境。本研究结果为植物-微生物原位修复碱性尾矿提供了依据。
