Phytotoxicity of environmental norfloxacin concentrations on the aquatic plant Spirodela polyrrhiza: Evaluation of growth parameters, photosynthetic toxicity and biochemical traits.

As an emerging pollutant, the increasing use of antibiotics in wastewater posed a serious threat to non-target organisms in the environment. Duckweed (Spirodela polyrrhiza) is a common higher aquatic plant broadly used in phytotoxicity tests for xenobiotic substances. The aim of this study was to evaluate the chronic toxicity of norfloxacin (NOR) on Spirodela polyrrhiza during 18 days of exposure. Our study investigated the addition of NOR into the medium with environment-related concentrations (0, 0.1, 10, and 1000 μg L). Subsequently, biomarkers of toxicity such as growth, pigment, chlorophyll fluorescence parameters, indicators of oxidative stress, and osmotic regulatory substances content were analyzed in duckweed. In response to NOR exposure, obvious chlorosis, declines in growth and photosynthetic pigment, and photosystem II inhibition were noted in a concentration dependent manner. Reactive oxygen species (ROS) and antioxidant activity content increased in the treated fronds, which indicated that oxidative stress was specifically affected by NOR exposure. A slight increase in osmotic regulatory substances in NOR treated setups than in the control represented the increasing stress resistance. These results suggest NOR exerts its toxic effects on the aquatic plant Spirodela polyrrhiza.