College of Biosystems Engineering and Food Science, Zhejiang University, Hang Zhou, 310058, Zhejiang, PR China.
Institute of Materials Science and Devices, School of Materials Science and Engineering, Suzhou University of Science and Technology, Suzhou, 215011, Jiangsu, PR China.
Anal Chim Acta. 2022 May 22;1208:339791. doi: 10.1016/j.aca.2022.339791. Epub 2022 Apr 23.
Oxidative stress of aquatic microorganisms under heavy metal stress is closely reflected by metabolite changes in cells but it is very difficult to study due to the fast metabolism process and severe in-situ measurements hurdle. Herein, the oxidative stress of cadmium on Euglena gracilis was systematically studied through multi-combined techniques. In particular, for the first time electrochemical approach was associated with Raman spectroscopy imaging to vividly to investigate temporal-spatially varied oxidative stress and its effects on cells metabolism, in which former real-time measured a volcanic relation of extracellular hydrogen peroxide versus the increase of cadmium stress, while the latter shows the corresponding metabolic changes by Raman imaging of single cells. This work builds a bridge to unravel the mechanism of cellular oxidative stress under harsh conditions in a more systematic and holistic approach, while holding a great promise to construct heavy metal biosensors precisely monitoring high heavy metal tolerance strains for environmental modification.
水生微生物在重金属胁迫下的氧化应激反应可以通过细胞内代谢物的变化来反映，但由于代谢过程较快，原位测量难度较大，因此很难对其进行研究。本研究采用多种组合技术系统地研究了镉胁迫对绿眼虫的氧化应激。特别是，首次将电化学方法与拉曼光谱成像相结合，生动地研究了时空变化的氧化应激及其对细胞代谢的影响，前者实时测量了细胞外过氧化氢与镉胁迫增加之间的火山关系，而后者则通过单细胞拉曼成像显示了相应的代谢变化。这项工作为在更系统和全面的方法下揭示恶劣条件下细胞氧化应激的机制架起了一座桥梁，同时也为构建重金属生物传感器提供了可能，从而精确监测具有高重金属耐受能力的菌株，以用于环境改良。
