基于磁性分子印迹粒子和金属离子的电化学检测牛奶中氨基糖苷类抗生素残留

Electrochemical detection of aminoglycoside antibiotics residuals in milk based on magnetic molecularly imprinted particles and metal ions.

机构信息

College of Mechanical and Electronic Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.

College of Chemistry and Pharmacy, Northwest A&F University, Yangling, Shaanxi 712100, China.

出版信息

Food Chem. 2022 Sep 30;389:133120. doi: 10.1016/j.foodchem.2022.133120. Epub 2022 Apr 29.

DOI:10.1016/j.foodchem.2022.133120

PMID:35526288

Abstract

This work proposed a cost-effective, simple, and highly sensitive magnetic molecularly imprinted particles (MMIPs) electrochemical sensor to indirectly detect kanamycin (KAN), tobramycin (TOB), and gentamicin (GEN). The MMIPs electrochemical sensor was prepared by a complex of metal ions and the MMIPs of rebinding the template, which modified on the magnetic glassy carbon electrode surface. Here, metal ions were used as the signal tracers and amplifiers of the MMIPs electrochemical sensor. Then the response peak currents of metal re-oxidized to metal ion was recorded by differential pulse voltammetry and used to monitor the level of aminoglycoside antibiotics. Under the optimal conditions, the MMIPs electrochemical sensors showed a high sensitivity toward KAN, TOB, and GEN with detection limits of 4.88, 1.28, and 1.07 nmol/L, respectively. Furthermore, the MMIPs electrochemical sensors showed high selectivity for determining KAN, TOB, and GEN, and they were successfully used to detect KAN, TOB, and GEN in milk.

摘要

本工作提出了一种经济、简单、高灵敏度的磁性分子印迹粒子（MMIPs）电化学传感器，用于间接检测卡那霉素（KAN）、妥布霉素（TOB）和庆大霉素（GEN）。MMIPs 电化学传感器是通过金属离子与 MMIPs 的复合物制备的，该复合物可重新结合模板并修饰在磁性玻碳电极表面。在这里，金属离子被用作 MMIPs 电化学传感器的信号示踪剂和放大器。然后，通过差分脉冲伏安法记录金属重新氧化为金属离子的响应峰电流，并用于监测氨基糖苷类抗生素的水平。在最佳条件下，MMIPs 电化学传感器对 KAN、TOB 和 GEN 具有高灵敏度，检测限分别为 4.88、1.28 和 1.07 nmol/L。此外，MMIPs 电化学传感器对 KAN、TOB 和 GEN 的测定具有高选择性，并成功用于牛奶中 KAN、TOB 和 GEN 的检测。