Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China; Key Laboratory of Forestry Genetics & Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037, China.
Bioresour Technol. 2022 Jul;355:127269. doi: 10.1016/j.biortech.2022.127269. Epub 2022 May 5.
Acid salts have been shown to catalyze xylan hydrolysis selectively and efficiently for xylooligosaccharides (XOS) production while using acid salts that are non-toxic and available as feed additives can avoid separation from resulting XOS-rich hydrolysates. There is no report on XOS production with sodium bisulfate (NaHSO) hydrolysis, of significance is that NaHSO as feed additive does not need to be separated. In this work, NaHSO hydrolysis was firstly employed to produce XOS from poplar. XOS yield of 42.7% was reached under optimal conditions of 0.04 mol/L NaHSO, 170 °C and 60 min. After hydrogen peroxide/acetic acid and sodium hydroxide treatments of NaHSO-pretreated poplar, high yields of glucose (92.0%) and xylose (91.3%) were obtained at a low cellulase dose of 5 FPU/g dry mass. NaHSO hydrolysis was a novel strategy to prepare XOS efficiently with simple operation steps, and XOS-rich hydrolysates could be potentially used as feed additives without NaHSO separation.
酸式盐已被证明可以在用于生产木低聚糖（XOS）时选择性和有效地催化木聚糖水解，而使用非毒性且可用作饲料添加剂的酸式盐可以避免与富含 XOS 的水解产物分离。目前还没有关于使用硫酸氢钠（NaHSO）水解生产 XOS 的报道，具有重要意义的是，作为饲料添加剂的 NaHSO 不需要分离。在这项工作中，首次采用 NaHSO 水解从杨木中生产 XOS。在 0.04 mol/L NaHSO、170°C 和 60 min 的最佳条件下，XOS 的得率达到了 42.7%。对 NaHSO 预处理后的杨木进行过氧化氢/乙酸和氢氧化钠处理后，在低纤维素酶用量 5 FPU/g 干质量的条件下，葡萄糖（92.0%）和木糖（91.3%）的得率均很高。NaHSO 水解是一种高效制备 XOS 的新策略，操作步骤简单，富含 XOS 的水解产物无需分离即可潜在用作饲料添加剂。
