Yang Qianqian, Ying Wenjun, Wen Peiyao, Zhu Junjun, Xu Yong, Zhang Junhua
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China; Key Laboratory of Forestry Genetics & Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037, China.
Bioresour Technol. 2021 Dec;342:125943. doi: 10.1016/j.biortech.2021.125943. Epub 2021 Sep 14.
Recently, xylo-oligosaccharides (XOS) production from lignocelluloses by organic acid catalysis has been widely reported. However, the effect of delignification of lignocelluloses on XOS production by organic acid catalysis was unclear, and lactic acid (LA) catalysis in XOS production from lignocelluloses has not been reported. In this work, the effect of delignification on XOS production from poplar by LA catalysis was investigated. Results demonstrated that hydrogen peroxide-acetic acid (HPAA) pretreatment removed 83.2% of lignin and retained 95.4% of xylan. After 2% LA catalysis (170 °C, 30 min), a high XOS yield of 42.7% was obtained from HPAA-LA-pretreated poplar. Lignin removal from poplar was positively correlated with XOS yield. Glucose yield of HPAA-LA-pretreated poplar by cellulase was 88.9%. Compared with LA-catalyzed poplar, the XOS and glucose production from HPAA-LA-pretreated poplar by cellulase increased by 1.4-fold and 6.8-fold, respectively. This work presents a novel strategy for efficient producing XOS and monosaccharides from poplar.
最近，关于通过有机酸催化从木质纤维素生产木寡糖（XOS）的报道颇多。然而，木质纤维素的脱木质素作用对有机酸催化生产XOS的影响尚不清楚，且尚未见关于乳酸（LA）催化从木质纤维素生产XOS的报道。在本研究中，考察了脱木质素对LA催化杨木生产XOS的影响。结果表明，过氧化氢-乙酸（HPAA）预处理去除了83.2%的木质素，保留了95.4%的木聚糖。经2% LA催化（170℃，30分钟）后，HPAA-LA预处理的杨木XOS产率高达42.7%。杨木的木质素去除率与XOS产率呈正相关。HPAA-LA预处理的杨木经纤维素酶作用后的葡萄糖产率为88.9%。与LA催化的杨木相比，HPAA-LA预处理的杨木经纤维素酶作用后XOS和葡萄糖的产量分别提高了1.4倍和6.8倍。本研究提出了一种从杨木高效生产XOS和单糖的新策略。
