Jinzhou Medical University, Jinzhou 121001, People's Republic of China.
Jinzhou Medical University, Jinzhou 121001, People's Republic of China.
Biomater Adv. 2022 Feb;133:112638. doi: 10.1016/j.msec.2021.112638. Epub 2022 Jan 5.
The complex wound environment and abnormalities in self-metabolism of diabetic skin defects lead to impaired angiogenesis, which can easily lead to bacterial infection and increased inflammation. Therefore, there is an urgent need for multifunctional composites with antimicrobial and pro-angiogenic properties to improve the current therapeutic outcomes of diabetic wounds. In this study, we described an EGCG-modified zinc oxide quantum dots (ZnO QDs) hydrogel (ZnO-EGCG@H) for the treatment of delayed diabetic wounds. In vitro antimicrobial assays showed that ZnO-EGCG@H effectively cleared 95.6% of MRSA and 97% of AmpE. coli with good bactericidal activity. Western blotting assay analysis showed that ZnO-EGCG@H downregulated trauma inflammatory factors (TNF-α, IL-6) by 46.9% and 57%, respectively, while upregulating 1.7-fold VEGF and 2-fold EGF, accelerating wound healing by reducing inflammatory response and promoting proliferation of vascular endothelial cells and skin epidermal cells. After 15 days of ZnO-EGCG@H treatment, the rate of skin lesion closure in rats was 96.3%, which was much better than that of 65.4% in the control group. Safety experiments showed that ZnO-EGCG@H was reassuringly biocompatible and harmless to vital organs. The obtained results suggested that ZnO-EGCG@H had the potential to be a safe and effective treatment method with a promising future as a diabetic wound treatment material.
复杂的伤口环境和糖尿病皮肤缺陷的自我代谢异常导致血管生成受损，容易导致细菌感染和炎症增加。因此，迫切需要具有抗菌和促血管生成特性的多功能复合材料来改善糖尿病伤口的当前治疗效果。在本研究中，我们描述了一种 EGCG 修饰的氧化锌量子点（ZnO QDs）水凝胶（ZnO-EGCG@H），用于治疗延迟性糖尿病伤口。体外抗菌试验表明，ZnO-EGCG@H 可有效清除 95.6%的 MRSA 和 97%的 AmpE。大肠杆菌具有良好的杀菌活性。Western blotting 分析表明，ZnO-EGCG@H 通过分别下调创伤炎症因子（TNF-α、IL-6）46.9%和 57%，而上调 1.7 倍的 VEGF 和 2 倍的 EGF，通过减少炎症反应和促进血管内皮细胞和皮肤表皮细胞的增殖来加速伤口愈合。在 ZnO-EGCG@H 治疗 15 天后，大鼠皮肤损伤的闭合率为 96.3%，明显优于对照组的 65.4%。安全性实验表明，ZnO-EGCG@H 具有令人放心的生物相容性，对重要器官无害。研究结果表明，ZnO-EGCG@H 具有成为一种安全有效的治疗方法的潜力，有望成为治疗糖尿病伤口的材料。
