• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Glucose stimulates the biosynthesis of rat I and II insulin to an equal extent in isolated pancreatic islets.

作者信息

Rhodes C J, Lucas C A, Halban P A

出版信息

FEBS Lett. 1987 May 4;215(1):179-82. doi: 10.1016/0014-5793(87)80137-0.

DOI:10.1016/0014-5793(87)80137-0
PMID:3552733
Abstract

The effects of glucose on insulin biosynthesis were studied by measuring the incorporation of radiolabelled amino acids into proinsulin/insulin in isolated rat islets. The islets were pulse labelled for 15 min with [3H]leucine (present in rat insulin I and II) or [35S]methionine (unique to rat insulin II) and then incubated for a 165 min post-label (chase) period during which the majority of labelled proinsulin was converted to insulin but under conditions whereby greater than 95% of radiolabelled proinsulin or insulin was retained in the islets. The newly synthesized, labelled, insulin was analyzed by high performance liquid chromatography. Rat I and II insulin biosynthesis was stimulated by 16.7 mM glucose to the same extent.

摘要

相似文献

1
Glucose stimulates the biosynthesis of rat I and II insulin to an equal extent in isolated pancreatic islets.
FEBS Lett. 1987 May 4;215(1):179-82. doi: 10.1016/0014-5793(87)80137-0.
2
Differential kinetics of rat insulin I and II processing in rat islets of Langerhans.
FEBS Lett. 1987 Nov 2;223(2):227-31. doi: 10.1016/0014-5793(87)80294-6.
3
Effect of interleukin-1 on the biosynthesis of proinsulin and insulin in isolated rat pancreatic islets.白细胞介素-1对离体大鼠胰岛中胰岛素原和胰岛素生物合成的影响。
Biomed Biochim Acta. 1988;47(4-5):305-9.
4
Biosynthesis of proinsulin and insulin in newborn rat pancreas. Interaction of glucose, cyclic AMP, somatostatin, and sulfonylureas on the (3H) leucine incorporation into immunoreactive insulin.新生大鼠胰腺中胰岛素原和胰岛素的生物合成。葡萄糖、环磷酸腺苷、生长抑素和磺脲类药物对（3H）亮氨酸掺入免疫反应性胰岛素的相互作用。
J Clin Invest. 1976 Jan;57(1):230-43. doi: 10.1172/JCI108264.
5
Alterations in regulation of insulin biosynthesis in pregnancy and starvation studied in isolated rat islets of langerhans.在分离的大鼠胰岛中研究妊娠和饥饿状态下胰岛素生物合成调节的变化。
Biochem J. 1977 Sep 15;166(3):501-7. doi: 10.1042/bj1660501.
6
Transcriptional and translational control of glucose-stimulated (pro)insulin biosynthesis.葡萄糖刺激的（前）胰岛素生物合成的转录和翻译控制
Eur J Biochem. 1980 Sep;110(2):499-505. doi: 10.1111/j.1432-1033.1980.tb04892.x.
7
Influence of vitamin D3 deficiency and 1,25 dihydroxyvitamin D3 on de novo insulin biosynthesis in the islets of the rat endocrine pancreas.维生素D3缺乏及1,25-二羟维生素D3对大鼠内分泌胰腺胰岛中胰岛素从头生物合成的影响。
J Endocrinol. 1999 Jan;160(1):87-95. doi: 10.1677/joe.0.1600087.
8
Biosynthesis of proinsulin and other islet cell proteins in pancreatic beta cells of the rat: a radioautographic evaluation of glucose effects in vitro.大鼠胰腺β细胞中胰岛素原及其他胰岛细胞蛋白的生物合成：体外葡萄糖效应的放射自显影评估
Diabetes. 1980 Oct;29(10):795-800. doi: 10.2337/diacare.20.10.795.
9
Effects of islet amyloid polypeptide (IAPP) on insulin biosynthesis or secretion in rat islets and mouse beta TC3 cells. Biosynthesis of IAPP in mouse beta TC3 cells.胰岛淀粉样多肽（IAPP）对大鼠胰岛和小鼠β TC3细胞中胰岛素生物合成或分泌的影响。小鼠β TC3细胞中IAPP的生物合成。
Diabetes Res Clin Pract. 1992 Jan;15(1):49-55. doi: 10.1016/0168-8227(92)90067-2.
10
Glucose regulated insulin biosynthesis in isolated rat pancreatic islets is accompanied by changes in proinsulin mRNA.在分离的大鼠胰岛中，葡萄糖调节的胰岛素生物合成伴随着胰岛素原信使核糖核酸的变化。
Diabetes Res. 1985 Mar;2(2):71-5.

引用本文的文献

1
A genetic screen identifies Crat as a regulator of pancreatic beta-cell insulin secretion.一项遗传筛选发现 Crat 是调节胰腺β细胞胰岛素分泌的一个调控因子。
Mol Metab. 2020 Jul;37:100993. doi: 10.1016/j.molmet.2020.100993. Epub 2020 Apr 13.
2
Islet beta cell failure in the 60% pancreatectomised obese hyperlipidaemic Zucker fatty rat: severe dysfunction with altered glycerolipid metabolism without steatosis or a falling beta cell mass.60%胰腺切除的肥胖高脂血症Zucker脂肪大鼠的胰岛β细胞功能衰竭：严重功能障碍伴甘油脂质代谢改变，无脂肪变性或β细胞数量减少。
Diabetologia. 2009 Jun;52(6):1122-32. doi: 10.1007/s00125-009-1317-8. Epub 2009 Mar 18.
3
Adaptation to supraphysiologic levels of insulin gene expression in transgenic mice: evidence for the importance of posttranscriptional regulation.
转基因小鼠对胰岛素基因表达超生理水平的适应性：转录后调控重要性的证据
J Clin Invest. 1993 Jul;92(1):272-80. doi: 10.1172/JCI116561.
4
Proinsulin processing in the regulated and the constitutive secretory pathway.胰岛素原在调节性分泌途径和组成型分泌途径中的加工过程。
Diabetologia. 1994 Sep;37 Suppl 2:S65-72. doi: 10.1007/BF00400828.
5
The intracellular handling of insulin-related peptides in isolated pancreatic islets. Evidence for differential rates of degradation of insulin and C-peptide.分离的胰岛中胰岛素相关肽的细胞内处理。胰岛素和C肽降解速率差异的证据。
Biochem J. 1988 Apr 1;251(1):23-30. doi: 10.1042/bj2510023.
6
Differential inhibitory action of cationic amino acids on protein synthesis in pancreatic rat islets.阳离子氨基酸对大鼠胰腺胰岛蛋白质合成的差异抑制作用。
Diabetologia. 1988 Apr;31(4):241-6. doi: 10.1007/BF00290592.
7
Partial diversion of a mutant proinsulin (B10 aspartic acid) from the regulated to the constitutive secretory pathway in transfected AtT-20 cells.在转染的AtT-20细胞中，突变胰岛素原（B10天冬氨酸）从调节性分泌途径部分转向组成型分泌途径。
Proc Natl Acad Sci U S A. 1989 Jun;86(11):4107-11. doi: 10.1073/pnas.86.11.4107.
8
High-performance liquid chromatography analysis of circulating insulins distinguishes between endogenous insulin production (a potential pitfall with streptozotocin diabetic rats) and islet xenograft function.
Diabetologia. 1990 Aug;33(8):457-61. doi: 10.1007/BF00405105.