Palace G P, Franke J E, Warden J T
FEBS Lett. 1987 May 4;215(1):58-62. doi: 10.1016/0014-5793(87)80113-8.
Comparative quantitative analysis of phylloquinone content and photochemically competent P-700 has been performed on photosystem I particles subjected to photolysis with ultraviolet irradiation. Nonirradiated control particles exhibit a phylloquinone/P-700 stoichiometry of 1.9 +/- 0.2. Photolysis of the photosystem I particles induces a progressive depletion of phylloquinone, however, photochemistry as assayed at room temperature by the photooxidation of P-700 is unaffected. These data are not consistent with the assignment of phylloquinone as a functional intermediate at room temperature between P-700 and the iron-sulfur clusters, center A and center B.
对经紫外线照射光解的光系统I颗粒进行了叶绿醌含量和具有光化学活性的P-700的比较定量分析。未照射的对照颗粒显示叶绿醌/P-700化学计量比为1.9±0.2。光系统I颗粒的光解导致叶绿醌逐渐消耗，然而，在室温下通过P-700的光氧化测定的光化学不受影响。这些数据与叶绿醌作为室温下P-700与铁硫簇（中心A和中心B）之间的功能中间体的说法不一致。
