[宫内生长迟缓大鼠基因表达与肝脏脂肪含量的相关性]
[Correlation of gene expression with hepatic fat content in rats with intrauterine growth retardation].
作者信息
Bian Jing, Chen Ping-Yang, Bian Du-Jun, He Xiao-Ri, Mutamba Alpha Kalonda, Wang Tao
机构信息
College of Life Sciences, Hunan Normal University, Changsha 410006, China.
出版信息
Zhongguo Dang Dai Er Ke Za Zhi. 2022 Apr 15;24(4):440-446. doi: 10.7499/j.issn.1008-8830.2110130.
OBJECTIVES
To study the correlation of the expression of in visceral adipose tissue and in liver tissue with hepatic fat content in rats with intrauterine growth retardation (IUGR).
METHODS
Pregnant rats were given a low-protein (10% protein) diet during pregnancy to establish a model of IUGR in neonatal rats. The pregnant rats in the control group were given a normal-protein (21% protein) diet during pregnancy. The neonatal rats were weighed and liver tissue was collected on day 1 and at weeks 3, 8, and 12 after birth, and visceral adipose tissue was collected at weeks 3, 8, and 12 after birth. The 3.0T H-magnetic resonance spectroscopy was used to measure hepatic fat content at weeks 3, 8, and 12 after birth. Real-time PCR was used to measure mRNA expression levels of in liver tissue and in visceral adipose tissue. Western blot was used to measure protein levels of in liver tissue and in visceral adipose tissue. A Pearson correlation analysis was performed to investigate the correlation of mRNA and protein expression of Lipin with hepatic fat content.
RESULTS
The IUGR group had significantly higher mRNA and protein expression levels of Lipin1 in visceral adipose tissue than the control group at weeks 3, 8, and 12 after birth (<0.05). Compared with the control group, the IUGR group had significantly lower mRNA and protein expression levels of Lipin2 in liver tissue on day 1 after birth and significantly higher mRNA and protein expression levels of Lipin2 at weeks 1, 3, 8, and 12 after birth (<0.05). At week 3 after birth, there was no significant difference in hepatic fat content between the IUGR and control groups (>0.05), while at weeks 8 and 12 after birth, the IUGR group had a significantly higher hepatic fat content than the control group (<0.05). The protein and mRNA expression levels of Lipin1 were positively correlated with hepatic fat content (=0.628 and 0.521 respectively; <0.05), and the protein and mRNA expression levels of Lipin2 were also positively correlated with hepatic fat content (=0.601 and 0.524 respectively; <0.05).
CONCLUSIONS
Upregulation of the mRNA and protein expression levels of Lipin1 in visceral adipose tissue and Lipin2 in liver tissue can increase hepatic fat content in rats with IUGR and may be associated with obesity in adulthood.
目的
研究宫内生长受限（IUGR）大鼠内脏脂肪组织和肝脏组织中Lipin的表达与肝脏脂肪含量的相关性。
方法
妊娠大鼠在孕期给予低蛋白（10%蛋白质）饮食以建立新生大鼠IUGR模型。对照组妊娠大鼠在孕期给予正常蛋白（21%蛋白质）饮食。新生大鼠在出生后第1天以及出生后3周、8周和12周称重并采集肝脏组织，在出生后3周、8周和12周采集内脏脂肪组织。采用3.0T氢磁共振波谱法测量出生后3周、8周和12周的肝脏脂肪含量。采用实时定量聚合酶链反应测量肝脏组织和内脏脂肪组织中Lipin的mRNA表达水平。采用蛋白质印迹法测量肝脏组织和内脏脂肪组织中Lipin的蛋白水平。进行Pearson相关分析以研究Lipin的mRNA和蛋白表达与肝脏脂肪含量的相关性。
结果
出生后3周、8周和12周，IUGR组内脏脂肪组织中Lipin1的mRNA和蛋白表达水平显著高于对照组（P<0.05）。与对照组相比，IUGR组出生后第1天肝脏组织中Lipin2的mRNA和蛋白表达水平显著降低，而出生后1周、3周、8周和12周Lipin2的mRNA和蛋白表达水平显著升高（P<0.05）。出生后3周，IUGR组和对照组的肝脏脂肪含量无显著差异（P>0.05），而出生后8周和12周，IUGR组的肝脏脂肪含量显著高于对照组（P<0.05）。Lipin1的蛋白和mRNA表达水平与肝脏脂肪含量呈正相关（分别为r=0.628和0.521；P<0.05），Lipin2的蛋白和mRNA表达水平也与肝脏脂肪含量呈正相关（分别为r=0.601和0.524；P<0.05）。
结论
IUGR大鼠内脏脂肪组织中Lipin1和肝脏组织中Lipin2的mRNA和蛋白表达水平上调可增加肝脏脂肪含量，可能与成年期肥胖有关。
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