Department of Digestive Internal Medicine, Lianyungang Hospital of Traditional Chinese Medicine, Lianyungang, Jiangsu, China.
Department of Digestive Internal Medicine, Lianyungang Hospital of Traditional Chinese Medicine, Lianyungang, Jiangsu, China;
Allergol Immunopathol (Madr). 2022 May 1;50(3):62-70. doi: 10.15586/aei.v50i3.584. eCollection 2022.
Momordica charantia exerts anti-inflammatory effect against ulcerative colitis. Momordica charantia polysaccharides (MCPs) attenuate gastritis through inhibition of ethanol-induced inflammatory response.
The role of MCPs in diarrhea-predominant irritable bowel syndrome (IBS-D) is investigated.
Chemical stimulation followed by acute and chronic pressure stimulation was used to establish rats model with IBS-D. The model rats were then administrated with MCPs. Defecation frequency, fecal water content and abdominal withdrawal reflex (AWR) score were then recorded. Pathologic changes in the colonic tissues were evaluated by hematoxylin and eosin staining. Inflammation was detected by ELISA and qRT-PCR, and immunohistochemistry was used to assess intestinal mucosal permeability.
First, IBS-D of mice wasIBS-D ratsmice exhibited many abnormal clinical manifestations, including increased frequency of defecation, fecal water content, and abdominal withdrawal reflex (AWR) score. Second, the mice were administrated with MCPs, which reduced frequency of defecation, fecal water content, and AWR score, and 100-mg/kg MCPs indicated therapeutic effect on IBS-D mice equivalent to rifaximin. Moreover, MCPs also ameliorated pathologic changes in the colonic tissues of IBS-D mice. Third, inflammatory response in IBS-D mice was also suppressed by MCPs through up-regulation of Interleukin (IL)-10, and down-regulation of tumor necrosis factor-α (TNF-α), Interleukin(IL)-1β, and IL-6. MCPs enhanced levels of occludin (OCLN) and zona occludens protein-1 (ZO-1) in IBS-D mice to improve intestinal mucosal permeability. Finally, phosphorylation of p65 in IBS-D mice was reduced by MCP treatment.
MCPs ameliorated intestinal permeability and repressed intestinal inflammation to attenuate IBS-D by inactivating nuclear factor kappa B (NF-κB) signaling.
苦瓜具有抗炎作用，可治疗溃疡性结肠炎。苦瓜多糖（MCPs）可通过抑制乙醇诱导的炎症反应来减轻胃炎。
研究 MCPs 在腹泻型肠易激综合征（IBS-D）中的作用。
采用化学刺激结合急性和慢性压力刺激的方法，建立 IBS-D 大鼠模型。然后给予 MCPs 治疗，记录排便频率、粪便含水量和腹壁退缩反射（AWR）评分。苏木精和伊红染色评估结肠组织的病理变化。酶联免疫吸附试验（ELISA）和 qRT-PCR 检测炎症，免疫组化评估肠黏膜通透性。
首先，IBS-D 模型鼠表现出许多异常的临床症状，包括排便频率增加、粪便含水量增加和 AWR 评分增加。其次，给予 MCPs 治疗后，IBS-D 模型鼠的排便频率、粪便含水量和 AWR 评分均降低，100mg/kg MCPs 对 IBS-D 模型鼠的治疗效果与利福昔明相当。此外，MCPs 还改善了 IBS-D 模型鼠的结肠组织病理变化。第三，MCPs 通过上调白细胞介素（IL）-10 并下调肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-1β 和 IL-6，抑制 IBS-D 模型鼠的炎症反应。MCPs 增加了 IBS-D 模型鼠紧密连接蛋白（OCLN）和闭合蛋白-1（ZO-1）的水平，改善了肠黏膜通透性。最后，MCP 处理可降低 IBS-D 模型鼠 p65 的磷酸化。
MCPs 通过抑制核因子-κB（NF-κB）信号通路改善肠黏膜通透性，抑制肠道炎症，从而减轻 IBS-D。
