异位移植大鼠心脏中的蛋白质合成

Protein synthesis in heterotopically transplanted rat hearts.

作者信息

Currie R W, Sharma V K, Stepkowski S M, Payce R F

出版信息

Exp Cell Biol. 1987;55(1):46-56. doi: 10.1159/000163394.

Abstract

This study examines protein synthesis in heterotopically transplanted rat hearts and several tissues of recipient rats. Donor hearts and recipient tissues synthesized many of the normally occurring proteins observed in tissues of unstressed rats. In addition, a stress-induced protein with a molecular mass of 71 kilodaltons was synthesized in donor heart, recipient heart and lung. Donor hearts incorporated more L-[35S]-methionine than did recipient hearts. Tissues of recipient rats also incorporated more label than the respective tissues of sham-recipient rats. These results suggest that ischemia, endured by the donor hearts during transplantation, induced these changes in protein synthesis.

摘要

本研究检测了异位移植大鼠心脏及受体大鼠多个组织中的蛋白质合成情况。供体心脏和受体组织合成了许多在未受应激大鼠组织中观察到的正常存在的蛋白质。此外，在供体心脏、受体心脏和肺中合成了一种分子量为71千道尔顿的应激诱导蛋白。供体心脏比受体心脏掺入更多的L-[35S]-甲硫氨酸。受体大鼠的组织也比假受体大鼠的相应组织掺入更多的放射性标记。这些结果表明，供体心脏在移植过程中所经历的缺血诱导了蛋白质合成的这些变化。