Gu Yun-Qiong, Shen Wen-Ying, Mi Yan, Jing Yan-Fang, Yuan Jing-Mei, Yu Peng, Zhu Xiao-Min, Hu Fei-Long
School of Environment and Life Science, Nanning Normal University Nanning 530001 Guangxi China.
Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Guangxi Normal University), Ministry of Education of China China.
RSC Adv. 2019 Nov 4;9(61):35671-35676. doi: 10.1039/c9ra06227k. eCollection 2019 Oct 31.
Herein, a dual-response fluorescent sensor, L, based on pyrazolopyrimidine was designed and developed for the simultaneous detection of Ni and Cu ions in the presence of other metal ions; the structural characterization of L was carried out by FTIR spectroscopy, NMR spectroscopy, HRMS and X-ray diffraction analysis. The sensor L effectively displayed fluorescence quenching towards the Ni and Cu ions with high sensitivity without interference from other metal ions. The results reveal that L binds to Ni and Cu in a 2 : 1 pattern, which matches well with the result of the Job's plot. The association constants of L with Ni and Cu were 3.2 × 10 M and 7.57 × 10 M, respectively. The detection limits (DLs) are down to 8.9 nM for Ni and 8.7 nM for Cu. The fluorescence imaging of L in T-24 cells was investigated because of the low cytotoxicity of L, indicating that L could be used to detect Ni and Cu in living cells.
在此，设计并开发了一种基于吡唑并嘧啶的双响应荧光传感器L，用于在其他金属离子存在的情况下同时检测镍离子和铜离子；通过傅里叶变换红外光谱、核磁共振光谱、高分辨质谱和X射线衍射分析对L进行了结构表征。传感器L对镍离子和铜离子有效表现出荧光猝灭，灵敏度高，不受其他金属离子干扰。结果表明，L以2∶1的模式与镍和铜结合，这与Job曲线的结果非常吻合。L与镍和铜的缔合常数分别为3.2×10⁶ M和7.57×10⁶ M。镍和铜的检测限分别低至8.9 nM和8.7 nM。由于L的细胞毒性低，研究了L在T-24细胞中的荧光成像，表明L可用于检测活细胞中的镍和铜。
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