伊朗临床和环境中暗色丝孢真菌及其亲缘种的分离与分子特征分析
Isolation and molecular characterization of clinical and environmental dematiaceous fungi and relatives from Iran.
作者信息
Shokoohi Gholamreza, Badali Hamid, Ahmadi Bahram, Satoh Kazuo, Nouripour-Sisakht Sadegh, Nikaeen Mahnaz, Gramishoar Mohsen, Jalalizand Nilufar, Kianipour Sahar, Mirhendi Hossein, Makimura Koichi
机构信息
Department of Parasitology and Mycology, Faculty of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran.
Zoonosis Research Center, Jahrom University of Medical Sciences, Jahrom, Iran.
出版信息
Curr Med Mycol. 2021 Sep;7(3):1-8. doi: 10.18502/cmm.7.3.7798.
BACKGROUND AND PURPOSE
The frequency and genetic diversity of black fungi in environmental and clinical settings have not been fully studied in Iran. This study aimed to identify and evaluate intra- and inter-species DNA sequence variation and also understand the phylogenetic relationships of melanized fungi and relatives isolated from different geographical regions of Iran.
MATERIALS AND METHODS
In total, 111 clinical and environmental strains of dematiaceous fungi were isolated, and their internal transcribed spacer ribosomal DNA (rDNA) regions were sequenced and analyzed.
RESULTS
An inter-species nucleotide sequence diversity rate of 1 to 464 nucleotides was observed between the species. Intra-species differences were found in the strains of and by 0-59, 0-22, 0-4, 0-4, 0-3, 0-2, 0-2, 0-2, 0-2, 0-1, and 0-1 nucleotide, respectively.
CONCLUSION
The internal transcribed spacer rDNA is useful for the discrimination of several taxa of dematiaceous fungi. However, a better understanding of the taxonomy of species of requires a larger rDNA region or a library of other gene sequences.
背景与目的
在伊朗，环境和临床环境中黑色真菌的频率和遗传多样性尚未得到充分研究。本研究旨在识别和评估种内和种间的DNA序列变异，并了解从伊朗不同地理区域分离出的黑化真菌及其亲缘种的系统发育关系。
材料与方法
总共分离出111株暗色丝孢菌的临床和环境菌株，并对其内部转录间隔区核糖体DNA（rDNA）区域进行测序和分析。
结果
观察到种间核苷酸序列多样性率为1至464个核苷酸。在 和 的菌株中分别发现种内差异为0 - 59、0 - 22、0 - 4、0 - 4、0 - 3、0 - 2、0 - 2、0 - 2、0 - 2、0 - 1和0 - 1个核苷酸。
结论
内部转录间隔区rDNA可用于鉴别几种暗色丝孢菌分类群。然而，要更好地了解 物种的分类学，需要更大的rDNA区域或其他基因序列文库。
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