GEO 数据库筛选结合体外实验研究 hsa_circ_0003570 在婴幼儿血管瘤中的作用机制。
GEO Database Screening Combined with In Vitro Experiments to Study the Mechanism of hsa_circ_0003570 in Infantile Hemangiomas.
机构信息
Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan 030032, China.
Fujian Children's Hospital; Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou 350000, China.
出版信息
Comput Math Methods Med. 2022 Apr 28;2022:5643742. doi: 10.1155/2022/5643742. eCollection 2022.
OBJECTIVE
In this study, we screened out a type of differentially expressed circular RNA in infantile hemangioma (IH) cells and analyzed the mechanism in the malignant biological behavior of IH.
METHODS
Based on the GSE98795, GSE100682, and GSE43742 datasets, differential expression analysis of circRNAs, microRNAs, and mRNAs was performed. The relative expression level of RNA was detected by quantitative real-time polymerase chain reaction (qRT-PCR). MTT assay, Transwell, flow cytometry analysis, and western blot were used to study the effects of hsa_circ_0003570, hsa-miR-138-5p, and RGS5 on the proliferation and apoptosis of hemangioma endothelial cells (HEMECs).
RESULTS
The hsa_circ_0003570 and mRNA were upregulated in HEMECs, but hsa-miR-138-5p was downregulated. Silencing of hsa_circ_0003570 inhibited the proliferation of HEMECs and promoted the apoptosis of HEMECs. The malignant biological behaviors of hsa_circ_0003570 on the proliferation and apoptosis of HEMECs were reversed by hsa-miR-138-5p. Hsa_circ_0003570 acted as the ceRNA of hsa-miR-138-5p and upregulated the expression of RGS5. Silencing of RGS5 inhibited the proliferation, migration, and invasion of HEMECs and promoted apoptosis.
CONCLUSION
Hsa_circ_0003570 promotes IH cell proliferation and inhibits IH cell apoptosis through hsa-miR-138-5p/RGS5 axis.
目的
本研究筛选出婴幼儿血管瘤（IH）细胞中差异表达的环状 RNA，并分析其在 IH 恶性生物学行为中的机制。
方法
基于 GSE98795、GSE100682 和 GSE43742 数据集，进行环状 RNA、微小 RNA 和信使 RNA 的差异表达分析。采用实时定量聚合酶链反应（qRT-PCR）检测 RNA 的相对表达水平。MTT 试验、Transwell、流式细胞术分析和 Western blot 用于研究 hsa_circ_0003570、hsa-miR-138-5p 和 RGS5 对血管内皮细胞（HEMECs）增殖和凋亡的影响。
结果
HEMECs 中 hsa_circ_0003570 和 mRNA 上调，但 hsa-miR-138-5p 下调。沉默 hsa_circ_0003570 抑制 HEMECs 的增殖并促进 HEMECs 的凋亡。hsa-miR-138-5p 逆转了 hsa_circ_0003570 对 HEMECs 增殖和凋亡的恶性生物学行为。hsa_circ_0003570 作为 hsa-miR-138-5p 的 ceRNA 并上调 RGS5 的表达。沉默 RGS5 抑制 HEMECs 的增殖、迁移和侵袭，促进凋亡。
结论
hsa_circ_0003570 通过 hsa-miR-138-5p/RGS5 轴促进 IH 细胞增殖并抑制 IH 细胞凋亡。
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