Liao Sha, Zhao Meimei, Luo Jing, Luo Kai, Wu Jingni, Liu Ruimin, Wang Shixiang, Jia Pu, Bai Yajun, Zheng Xiaohui
Key Laboratory of Resource Biology and Biotechnology in Western China, Northwest University Xi'an 710069 China
School of Pharmacy, Shaanxi Institute of International Trade & Commerce Xi'an 712046 China.
RSC Adv. 2019 Aug 15;9(44):25569-25575. doi: 10.1039/c9ra02978h. eCollection 2019 Aug 13.
Herein, novel luminol functional gold nanoparticles (lum-AuNPs) were quickly prepared in an alkaline luminol solution with HAuCl, which had the unique characteristics of uniform size and excellent luminescence properties. A self-made flow injection-chemiluminescence (FI-CL) system was established to study the interaction between pepsin (Pep) and five alkaloids (anisodamine, berberine, reserpine, jatrorrhizine and matrine) using lum-AuNPs as the CL probe. Based on the abovementioned home-made CL system, the possible interaction mechanisms of Pep with five alkaloids have been comprehensively discussed by molecular docking simulation, chemical thermodynamics and kinetic studies. The results indicated that there were obvious CL enhancement and inhibition effects on the lum-AuNPs CL system for the Pep and the complex of Pep/alkaloids, respectively. The possible mechanism for the interaction of Pep-five alkaloids was mainly mediated by the hydrophobic force. The binding constant and binding site for the Pep-alkaloid interaction are consistent with the list of Ber > Res > Ani, Jat > Mat, which is relative to the potential of groups of alkaloids interacting with the active site of Pep.
在此，通过在碱性鲁米诺溶液中用氯金酸快速制备了新型鲁米诺功能化金纳米粒子（lum-AuNPs），其具有尺寸均匀和发光性能优异的独特特性。建立了一种自制的流动注射化学发光（FI-CL）系统，以鲁米诺功能化金纳米粒子作为化学发光探针，研究胃蛋白酶（Pep）与五种生物碱（山莨菪碱、黄连素、利血平、药根碱和苦参碱）之间的相互作用。基于上述自制的化学发光系统，通过分子对接模拟、化学热力学和动力学研究，全面探讨了胃蛋白酶与五种生物碱可能的相互作用机制。结果表明，胃蛋白酶及其与生物碱的复合物对鲁米诺功能化金纳米粒子化学发光系统分别有明显的增强和抑制作用。胃蛋白酶与五种生物碱相互作用的可能机制主要由疏水力介导。胃蛋白酶-生物碱相互作用的结合常数和结合位点与黄连素＞利血平＞山莨菪碱、药根碱＞苦参碱的顺序一致，这与生物碱基团与胃蛋白酶活性位点相互作用的潜力有关。
