Li Siying, Pan Yangang, Teng Honggang, Shan Yuping, Yang Guocheng, Wang Hongda
School of Chemistry and Life Science, Advanced Institute of Materials Science, Changchun University of Technology, Changchun, 130012 P. R. China.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 P. R. China.
Chem Res Chin Univ. 2022;38(3):838-842. doi: 10.1007/s40242-022-2069-y. Epub 2022 May 2.
The rabies virus is a neurotropic virus that causes fatal diseases in humans and animals. Although studying the interactions between a single rabies virus and the cell membrane is necessary for understanding the pathogenesis, the internalization dynamic mechanism of single rabies virus in living cells remains largely elusive. Here, we utilized a novel force tracing technique based on atomic force microscopy(AFM) to record the process of single viral entry into host cell. We revealed that the force of the rabies virus internalization distributed at (65±25) pN, and the time was identified by two peaks with spacings of (237.2±59.1) and (790.3±134.4) ms with the corresponding speed of 0.12 and 0.04 µm/s, respectively. Our results provide insight into the effects of viral shape during the endocytosis process. This report will be meaningful for understanding the dynamic mechanism of rabies virus early infection.
Supplementary material is available in the online version of this article at 10.1007/s40242-022-2069-y.
狂犬病病毒是一种嗜神经病毒，可导致人类和动物患上致命疾病。尽管研究单个狂犬病病毒与细胞膜之间的相互作用对于理解发病机制是必要的，但单个狂犬病病毒在活细胞中的内化动力学机制在很大程度上仍不清楚。在这里，我们利用基于原子力显微镜（AFM）的新型力追踪技术来记录单个病毒进入宿主细胞的过程。我们发现，狂犬病病毒内化的力分布在（65±25）皮牛，时间由两个峰值确定，间距分别为（237.2±59.1）和（790.3±134.4）毫秒，相应速度分别为0.12和0.04微米/秒。我们的结果为内吞过程中病毒形状的影响提供了见解。本报告对于理解狂犬病病毒早期感染的动力学机制具有重要意义。
补充材料可在本文的在线版本中获取，链接为10.1007/s40242-022-2069-y。
