时间聚焦显微镜中的远程聚焦
Remote Focusing in a Temporal Focusing Microscope.
作者信息
Durst Michael E, Yurak Samuel, Moscatelli Joseph, Linhares Isabel, Vargas Ruben
机构信息
Department of Physics, Middlebury College, Middlebury, VT 05753, USA.
出版信息
OSA Contin. 2021 Nov 15;4(11):2757-2770. doi: 10.1364/osac.443116. Epub 2021 Oct 27.
Abstract
In a temporal focusing microscope, dispersion can remotely shift the temporal focal plane axially, but only a single depth can be in focus at a time on a fixed camera. In this paper, we demonstrate remote focusing in a temporal focusing microscope. Dispersion tuning with an electrically tunable lens (ETL) in a 4 pulse shaper scans the excitation plane axially, and another ETL in the detection path keeps the shifted excitation plane in focus on the camera. Image stacks formed using two ETLs versus a traditional stage scan are equivalent.
摘要
在时间聚焦显微镜中，色散可以轴向远程移动时间焦平面，但在固定相机上一次只能有一个深度处于聚焦状态。在本文中，我们展示了时间聚焦显微镜中的远程聚焦。在四脉冲整形器中使用电可调透镜（ETL）进行色散调谐可轴向扫描激发平面，检测路径中的另一个ETL使移位后的激发平面在相机上保持聚焦。使用两个ETL形成的图像堆栈与传统的载物台扫描等效。
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